RRC ID 59316
著者 Ayuda-Durán B, González-Manzano S, Miranda-Vizuete A, Dueñas M, Santos-Buelga C, González-Paramás AM.
タイトル Epicatechin modulates stress-resistance in C. elegans via insulin/IGF-1 signaling pathway.
ジャーナル PLoS One
Abstract The nematode Caenorhabditis elegans has been used to examine the influence of epicatechin (EC), an abundant flavonoid in the human diet, in some stress biomarkers (ROS production, lipid peroxidation and protein carbonylation). Furthermore, the ability of EC to modulate the expression of some key genes in the insulin/IGF-1 signaling pathway (IIS), involved in longevity and oxidative or heat shock stress response, has also been explored. The final aim was to contribute to the elucidation of the mechanisms involved in the biological effects of flavonoids. The results showed that EC-treated wild-type C. elegans exhibited increased survival and reduced oxidative damage of biomolecules when submitted to thermal stress. EC treatment led to a moderate elevation in ROS levels, which might activate endogenous mechanisms of defense protecting against oxidative insult. The enhanced stress resistance induced by EC was found to be mediated through the IIS pathway, since assays in daf-2, age-1, akt-1, akt-2, sgk-1, daf-16, skn-1 and hsf-1 loss of function mutant strains failed to show any heat-resistant phenotype against thermal stress when treated with EC. Consistently, EC treatment upregulated the expression of some stress resistance associated genes, such as gst-4, hsp-16.2 and hsp-70, which are downstream regulated by the IIS pathway.
巻・号 14(1)
ページ e0199483
公開日 2019-1-1
DOI 10.1371/journal.pone.0199483
PII PONE-D-18-16837
PMID 30689636
PMC PMC6349306
MeSH Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Catechin / pharmacology* Insulin / genetics Insulin / metabolism* Insulin-Like Growth Factor I / genetics Insulin-Like Growth Factor I / metabolism* Lipid Peroxidation / drug effects Oxidative Stress / drug effects* Oxidative Stress / genetics Reactive Oxygen Species / metabolism Signal Transduction / drug effects* Signal Transduction / genetics
IF 2.776
引用数 11
リソース情報
線虫 tm812