論文 - 詳細
| RRC ID | 59381 |
|---|---|
| 著者 | Mentink RA, Rella L, Radaszkiewicz TW, Gybel T, Betist MC, Bryja V, Korswagen HC. |
| タイトル | The planar cell polarity protein VANG-1/Vangl negatively regulates Wnt/β-catenin signaling through a Dvl dependent mechanism. |
| ジャーナル | PLoS Genet |
| Abstract |
Van Gogh-like (Vangl) and Prickle (Pk) are core components of the non-canonical Wnt planar cell polarity pathway that controls epithelial polarity and cell migration. Studies in vertebrate model systems have suggested that Vangl and Pk may also inhibit signaling through the canonical Wnt/β-catenin pathway, but the functional significance of this potential cross-talk is unclear. In the nematode C. elegans, the Q neuroblasts and their descendants migrate in opposite directions along the anteroposterior body axis. The direction of these migrations is specified by Wnt signaling, with activation of canonical Wnt signaling driving posterior migration, and non-canonical Wnt signaling anterior migration. Here, we show that the Vangl ortholog VANG-1 influences the Wnt signaling response of the Q neuroblasts by negatively regulating canonical Wnt signaling. This inhibitory activity depends on a carboxy-terminal PDZ binding motif in VANG-1 and the Dishevelled ortholog MIG-5, but is independent of the Pk ortholog PRKL-1. Moreover, using Vangl1 and Vangl2 double mutant cells, we show that a similar mechanism acts in mammalian cells. We conclude that cross-talk between VANG-1/Vangl and the canonical Wnt pathway is an evolutionarily conserved mechanism that ensures robust specification of Wnt signaling responses. |
| 巻・号 | 14(12) |
| ページ | e1007840 |
| 公開日 | 2018-12-1 |
| DOI | 10.1371/journal.pgen.1007840 |
| PII | PGENETICS-D-18-00816 |
| PMID | 30532125 |
| PMC | PMC6307821 |
| MeSH | Animals Animals, Genetically Modified Body Patterning / genetics Body Patterning / physiology Caenorhabditis elegans / cytology* Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Lineage Cell Polarity / genetics Cell Polarity / physiology Dishevelled Proteins / genetics Dishevelled Proteins / metabolism Genes, Helminth Homeodomain Proteins / genetics Homeodomain Proteins / metabolism Intracellular Signaling Peptides and Proteins / genetics Intracellular Signaling Peptides and Proteins / metabolism Mutation Neural Stem Cells / cytology Neural Stem Cells / metabolism Phosphoproteins / genetics Phosphoproteins / metabolism* Transcription Factors / genetics Transcription Factors / metabolism Wnt Signaling Pathway / genetics Wnt Signaling Pathway / physiology* beta Catenin / genetics beta Catenin / metabolism |
| IF | 5.224 |
| 引用数 | 7 |
| リソース情報 | |
| 線虫 | tm1422 |