論文 - 詳細
| RRC ID | 59692 |
|---|---|
| 著者 | Tong L, Matsuura E, Takahashi M, Nagano T, Kawazu K. |
| タイトル | Effects of Anti-Glaucoma Prostaglandin Ophthalmic Solutions on Cultured Human Corneal Epithelial Cells. |
| ジャーナル | Curr Eye Res |
| Abstract |
Purpose: We compare the cytotoxicity of anti-glaucoma prostaglandin ophthalmic solutions on human corneal epithelial cells and elucidate mechanisms of toxicity. Methods: Cell viability was examined using MTS assay, and morphological changes of the cells were observed. Induction of necrosis/apoptosis was measured by colorimetric caspase assay. The production of Reactive oxygen species (ROS) and release of cytokines were analyzed using 2', 7'-dichlorodihydrofluorescein diacetate and bead-based indirect immunofluorescent assay, respectively. Results: Xalatan, Lumigan 0.01%, and Lumigan 0.03% decreased cell viability and induced morphological changes. Xalatan and Lumigan 0.01% induced necrosis. Xalatan, Lumigan 0.01%, Lumigan 0.03%, and Taflotan stimulated ROS production. Travatan and Lumigan 0.03% increased concentrations of Interleukin (IL)-6 and IL-8 in culture media. Conclusions: Xalatan and Lumigan 0.01% ophthalmic solutions demonstrated potent cytotoxicity compared with Lumigan 0.03%, Travatan, Taflotan, and Taflotan UD. Taflotan UD, compared to Taflotan 0.0015%, induced less oxidative stress and apoptotic signalling. The cytotoxicity might be partly associated with benzalkonium chloride. |
| 巻・号 | 44(8) |
| ページ | 856-862 |
| 公開日 | 2019-8-1 |
| DOI | 10.1080/02713683.2019.1597127 |
| PMID | 30884982 |
| MeSH | Antihypertensive Agents / pharmacology* Bimatoprost / pharmacology Cell Survival Cells, Cultured Cytokines / metabolism Epithelium, Corneal / drug effects* Epithelium, Corneal / metabolism Epithelium, Corneal / pathology Fluorescent Antibody Technique, Indirect Glaucoma / drug therapy Humans Latanoprost / pharmacology* Ophthalmic Solutions Prostaglandins F / pharmacology* Reactive Oxygen Species / metabolism Travoprost / pharmacology* |
| IF | 1.672 |
| 引用数 | 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HCE-T(RCB2280) |