RRC ID 59853
著者 Li J, Han G, Ma M, Wei G, Shi X, Guo Z, Li T, Meng H, Cao Y, Liu X.
タイトル Xanthan Gum Ameliorates Osteoarthritis and Mitigates Cartilage Degradation via Regulation of the Wnt3a/β-Catenin Signaling Pathway.
ジャーナル Med Sci Monit
Abstract BACKGROUND Osteoarthritis (OA) is a joint disease characterized by articular cartilage degeneration and inflammation. We have previously clarified that a xanthan gum (XG) preparation exerts ameliorating effect on a rabbit OA model by regulating matrix metalloproteinase (MMP)-1 and MMP-3, which are critical proteins in the Wnt3a/ß-catenin pathway. Thus, it is reasonable to predict that the Wnt3a/ß-catenin pathway is involved in the treatment of OA with XG. MATERIAL AND METHODS The effect of XG in OA model animals were observed by hematoxylin and eosin staining (HE), Safranin O staining, and Fast Green staining. Articular cartilage degradation on the medial plateau sides was quantified using the modified Pritzker OARSI score. The levels of IL-6, TNF-alpha, and IL-1ß in synovial fluid were determined with ELISA. The protective effect of XG in rat chondrocytes was assessed by CCK8 assay. Moreover, activation of the Wnt3a/ß-catenin pathway and the expression of MMP13, ADAMTS5, aggrecan, and collagen II under the influence of XG was measured by Western blot and qRT-PCR. RESULTS Our results showed that XG reduced the OARSI score and the concentration of inflammatory cytokines in OA after intra-articular injection. XG acted on Wnt3a/ß-catenin in ATDC5 cells in a dose-dependent manner and exhibited a protective effect. XG also decreased the expression of MMP13 and ADAMTS5 and rescued the inhibition of aggrecan and collagen II expression in SNP-stimulated chondrocytes. CONCLUSIONS These results indicate that the effects of XG are related to the Wnt3a/ß-catenin pathway and XG suppresses matrix degradation by inhibiting the expression of MMPs and ADAMTS and promotes aggrecan and collagen II content in the ECM, indicating its favorable potential for use in OA therapy.
巻・号 25
ページ 7488-7498
公開日 2019-10-6
DOI 10.12659/MSM.916092
PII 916092
PMID 31587011
PMC PMC6792505
MeSH ADAMTS5 Protein / metabolism Animals Cartilage / drug effects Cartilage / metabolism Cartilage / pathology Cartilage, Articular / drug effects Cartilage, Articular / metabolism Cartilage, Articular / pathology Cell Line Cells, Cultured Chondrocytes / drug effects Chondrocytes / metabolism Disease Models, Animal Inflammation / metabolism Male Matrix Metalloproteinase 13 / metabolism Osteoarthritis / drug therapy* Osteoarthritis / metabolism Osteoarthritis / pathology Polysaccharides, Bacterial / pharmacology* Rabbits Rats Rats, Sprague-Dawley Synovial Fluid / drug effects Synovial Fluid / metabolism Wnt Signaling Pathway / drug effects* Wnt3A Protein / metabolism beta Catenin / metabolism
IF 1.918
引用数 0
リソース情報
ヒト・動物細胞 ATDC5(RCB0565)