論文 - 詳細
| RRC ID | 60054 |
|---|---|
| 著者 | Chen H, Liang Q, Zhou X, Wang X. |
| タイトル | Preparative separation of the flavonoid fractions from Periploca forrestii Schltr. ethanol extracts using macroporous resin combined with HPLC analysis and evaluation of their biological activities. |
| ジャーナル | J Sep Sci |
| Abstract |
A preparative separation method using macroporous absorptive resin coupled with high-performance liquid chromatography was developed for the separation of six fractions of the 80% ethanol extract of Periploca forrestii Schltr. The six ethanol fractions (5-95; A, B, C, D, E, and F) obtained were carefully analyzed to locate the corresponding peaks in the high-performance liquid chromatography chromatogram of the total extract, which was established in a previous study. Furthermore, the biological activities, including antioxidant activities, acetyl cholinesterase inhibitory capacities, antihyaluronidase activities, and anti-inflammatory effects, were evaluated in MH7A cells. The results demonstrated that fraction E could significantly prevent oxidation and inhibit hyaluronidase and acetyl cholinesterase. Finally, the main flavonoids in fractions A and E from P. forrestii Schltr. were purified, and the compounds were identified as chlorogenic acid, quercetin-3-O-α-L-arabinopyranoside, and quercetin-7-O-β-D-glucopyranoside. The chemical structures were confirmed by mass spectrometry and nuclear magnetic resonance spectroscopy. Furthermore, the inhibitory effects of these compounds against complete Freund's adjuvant-induced secondary immune arthritis in rats were evaluated. |
| 巻・号 | 42(3) |
| ページ | 650-661 |
| 公開日 | 2019-2-1 |
| DOI | 10.1002/jssc.201800422 |
| PMID | 30461196 |
| MeSH | Acetylcholinesterase / metabolism* Animals Anti-Inflammatory Agents, Non-Steroidal / chemistry Anti-Inflammatory Agents, Non-Steroidal / isolation & purification Anti-Inflammatory Agents, Non-Steroidal / pharmacology* Antioxidants / chemistry Antioxidants / isolation & purification Antioxidants / pharmacology* Arthritis, Rheumatoid / chemically induced Arthritis, Rheumatoid / drug therapy* Biphenyl Compounds / antagonists & inhibitors Cell Line Chromatography, High Pressure Liquid Enzyme Inhibitors / chemistry Enzyme Inhibitors / isolation & purification Enzyme Inhibitors / pharmacology* Ethanol / chemistry Flavonoids / chemistry Flavonoids / isolation & purification Flavonoids / pharmacology Freund's Adjuvant / antagonists & inhibitors Freund's Adjuvant / pharmacology Humans Hyaluronoglucosaminidase / antagonists & inhibitors* Hyaluronoglucosaminidase / metabolism Particle Size Periploca / chemistry Picrates / antagonists & inhibitors Plant Extracts / chemistry Plant Extracts / isolation & purification Plant Extracts / pharmacology Porosity Rats Rats, Sprague-Dawley Resins, Plant / chemistry Surface Properties |
| IF | 2.878 |
| 引用数 | 5 |
| リソース情報 | |
| ヒト・動物細胞 | MH7A(RCB1512) |