| RRC ID |
601
|
| 著者 |
Tohge T, Nishiyama Y, Hirai MY, Yano M, Nakajima J, Awazuhara M, Inoue E, Takahashi H, Goodenowe DB, Kitayama M, Noji M, Yamazaki M, Saito K.
|
| タイトル |
Functional genomics by integrated analysis of metabolome and transcriptome of Arabidopsis plants over-expressing an MYB transcription factor.
|
| ジャーナル |
Plant J
|
| Abstract |
The integration of metabolomics and transcriptomics can provide precise information on gene-to-metabolite networks for identifying the function of unknown genes unless there has been a post-transcriptional modification. Here, we report a comprehensive analysis of the metabolome and transcriptome of Arabidopsis thaliana over-expressing the PAP1 gene encoding an MYB transcription factor, for the identification of novel gene functions involved in flavonoid biosynthesis. For metabolome analysis, we performed flavonoid-targeted analysis by high-performance liquid chromatography-mass spectrometry and non-targeted analysis by Fourier-transform ion-cyclotron mass spectrometry with an ultrahigh-resolution capacity. This combined analysis revealed the specific accumulation of cyanidin and quercetin derivatives, and identified eight novel anthocyanins from an array of putative 1800 metabolites in PAP1 over-expressing plants. The transcriptome analysis of 22,810 genes on a DNA microarray revealed the induction of 38 genes by ectopic PAP1 over-expression. In addition to well-known genes involved in anthocyanin production, several genes with unidentified functions or annotated with putative functions, encoding putative glycosyltransferase, acyltransferase, glutathione S-transferase, sugar transporters and transcription factors, were induced by PAP1. Two putative glycosyltransferase genes (At5g17050 and At4g14090) induced by PAP1 expression were confirmed to encode flavonoid 3-O-glucosyltransferase and anthocyanin 5-O-glucosyltransferase, respectively, from the enzymatic activity of their recombinant proteins in vitro and results of the analysis of anthocyanins in the respective T-DNA-inserted mutants. The functional genomics approach through the integration of metabolomics and transcriptomics presented here provides an innovative means of identifying novel gene functions involved in plant metabolism.
|
| 巻・号 |
42(2)
|
| ページ |
218-35
|
| 公開日 |
2005-4-1
|
| DOI |
10.1111/j.1365-313X.2005.02371.x
|
| PII |
TPJ2371
|
| PMID |
15807784
|
| MeSH |
Arabidopsis / genetics
Arabidopsis / metabolism*
Arabidopsis Proteins / metabolism
Chromosome Mapping
Chromosomes, Plant
Down-Regulation
Flavonols / biosynthesis*
Gene Expression / physiology*
Gene Expression Profiling
Genomics*
Pancreatitis-Associated Proteins
Phylogeny
Plant Leaves / physiology
Transcription Factors / genetics*
Up-Regulation
|
| IF |
6.141
|
| 引用数 |
594
|
|
WOS 分野
|
PLANT SCIENCES
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
pda07289 |
