論文 - 詳細
| RRC ID | 6026 |
|---|---|
| 著者 | Mizushima N, Yamamoto A, Matsui M, Yoshimori T, Ohsumi Y. |
| タイトル | In vivo analysis of autophagy in response to nutrient starvation using transgenic mice expressing a fluorescent autophagosome marker. |
| ジャーナル | Mol Biol Cell |
| Abstract |
Macroautophagy mediates the bulk degradation of cytoplasmic components. It accounts for the degradation of most long-lived proteins: cytoplasmic constituents, including organelles, are sequestered into autophagosomes, which subsequently fuse with lysosomes, where degradation occurs. Although the possible involvement of autophagy in homeostasis, development, cell death, and pathogenesis has been repeatedly pointed out, systematic in vivo analysis has not been performed in mammals, mainly because of a limitation of monitoring methods. To understand where and when autophagy occurs in vivo, we have generated transgenic mice systemically expressing GFP fused to LC3, which is a mammalian homologue of yeast Atg8 (Aut7/Apg8) and serves as a marker protein for autophagosomes. Fluorescence microscopic analyses revealed that autophagy is differently induced by nutrient starvation in most tissues. In some tissues, autophagy even occurs actively without starvation treatments. Our results suggest that the regulation of autophagy is organ dependent and the role of autophagy is not restricted to the starvation response. This transgenic mouse model is a useful tool to study mammalian autophagy. |
| 巻・号 | 15(3) |
| ページ | 1101-11 |
| 公開日 | 2004-3-1 |
| DOI | 10.1091/mbc.e03-09-0704 |
| PII | E03-09-0704 |
| PMID | 14699058 |
| PMC | PMC363084 |
| MeSH | Animals Autophagy / physiology* Autophagy-Related Protein 8 Family Green Fluorescent Proteins Luminescent Proteins / metabolism Lysosomes / metabolism* Mice Mice, Transgenic Microscopy, Immunoelectron Microtubule-Associated Proteins / genetics Phagosomes / metabolism* Saccharomyces cerevisiae Proteins / genetics Starvation* Tissue Distribution Tumor Cells, Cultured Vacuoles / metabolism* |
| IF | 3.791 |
| 引用数 | 1631 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 55 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC00806 |