RRC ID 60400
著者 Nakata H, Kanda H, Nakakita Y, Kaneko T, Tsuchiya Y.
タイトル Prevotella cerevisiae sp. nov., beer-spoilage obligate anaerobic bacteria isolated from brewery wastewater.
ジャーナル Int J Syst Evol Microbiol
Abstract Two obligate anaerobic, Gram-stain-negative, non-spore-forming bacilli (strains SBC 8034T and SBC 8065) were isolated from brewery wastewater. Cells of the two strains were rod-shaped and 0.8×2-5 µm in size. Strains SBC 8034T and SBC 8065 did not grow on Columbia agar or tryptic soy agar II with 5 % sheep blood, brain-heart infusion agar or chocolate agar, but did grow on peptone-yeast-glucose agar and de Man, Rogosa and Sharpe agar using beer instead of water. The organisms produced acetic acid and succinic acid as the major metabolic end-products. Phylogenetic analysis based on 16S rRNA gene sequences revealed that both strains are clearly distinct from all recognized species within the genus Prevotella, but belong to the same species (DDH=85 %). Based on 16S rRNA and hsp60 gene sequencing, along with phenotypic, chemical and biochemical properties, strains SBC 8034T and SBC 8065 were considered to represent a novel species within the genus Prevotella, for which the name Prevotellacerevisiae sp. nov. is proposed. Strain SBC 8034T (=DSM 100619T=JCM 30867T) is the type strain of the proposed novel species.
巻・号 69(6)
ページ 1789-1793
公開日 2019-6-1
DOI 10.1099/ijsem.0.003393
PMID 30990398
MeSH Bacteria, Anaerobic / genetics Bacterial Typing Techniques Base Composition Beer / microbiology* DNA, Bacterial / genetics Fatty Acids / chemistry Food Microbiology Genes, Bacterial Phylogeny* Prevotella / classification* Prevotella / isolation & purification RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Wastewater / microbiology*
IF 2.166
引用数 2
リソース情報
一般微生物 JCM30867