RRC ID |
60400
|
著者 |
Nakata H, Kanda H, Nakakita Y, Kaneko T, Tsuchiya Y.
|
タイトル |
Prevotella cerevisiae sp. nov., beer-spoilage obligate anaerobic bacteria isolated from brewery wastewater.
|
ジャーナル |
Int J Syst Evol Microbiol
|
Abstract |
Two obligate anaerobic, Gram-stain-negative, non-spore-forming bacilli (strains SBC 8034T and SBC 8065) were isolated from brewery wastewater. Cells of the two strains were rod-shaped and 0.8×2-5 µm in size. Strains SBC 8034T and SBC 8065 did not grow on Columbia agar or tryptic soy agar II with 5 % sheep blood, brain-heart infusion agar or chocolate agar, but did grow on peptone-yeast-glucose agar and de Man, Rogosa and Sharpe agar using beer instead of water. The organisms produced acetic acid and succinic acid as the major metabolic end-products. Phylogenetic analysis based on 16S rRNA gene sequences revealed that both strains are clearly distinct from all recognized species within the genus Prevotella, but belong to the same species (DDH=85 %). Based on 16S rRNA and hsp60 gene sequencing, along with phenotypic, chemical and biochemical properties, strains SBC 8034T and SBC 8065 were considered to represent a novel species within the genus Prevotella, for which the name Prevotellacerevisiae sp. nov. is proposed. Strain SBC 8034T (=DSM 100619T=JCM 30867T) is the type strain of the proposed novel species.
|
巻・号 |
69(6)
|
ページ |
1789-1793
|
公開日 |
2019-6-1
|
DOI |
10.1099/ijsem.0.003393
|
PMID |
30990398
|
MeSH |
Bacteria, Anaerobic / genetics
Bacterial Typing Techniques
Base Composition
Beer / microbiology*
DNA, Bacterial / genetics
Fatty Acids / chemistry
Food Microbiology
Genes, Bacterial
Phylogeny*
Prevotella / classification*
Prevotella / isolation & purification
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Wastewater / microbiology*
|
IF |
2.166
|
引用数 |
2
|
リソース情報 |
一般微生物 |
JCM30867 |