| RRC ID |
60468
|
| Author |
Huang SP, Chen TY, Chen JS, Wang LT, Huang L, Lin ST, Wei CL, Lin S, Wang PL, Chen YM, Shieh WY.
|
| Title |
Dongshaea marina gen. nov., sp. nov., a facultatively anaerobic marine bacterium that ferments glucose with gas production.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
Two isolates of heterotrophic, facultatively anaerobic, marine bacteria, designated DM1 and DM2T, were recovered from a lagoon sediment sample of Dongsha Island, Taiwan. Cells were Gram-reaction-negative rods. Nearly all of the cells were non-motile and non-flagellated during the late exponential to early stationary phase of growth, while a few of the cells exhibited motility with monotrichous flagellation. The two isolates required NaCl for growth and grew optimally at about 30 °C, 2-3 % NaCl and pH 7-8. They grew aerobically and could achieve anaerobic growth by fermenting d-glucose or other carbohydrates with production of acids and the gases, including CO2 and H2. Ubiquinone Q-8 was the only respiratory quinone. Cellular fatty acids were predominated by C16 : 0, C18 : 1ω7c and C16 : 1ω7c. The major polar lipid was phosphatidylethanolamine. Strains DM1 and DM2T had DNA G+C contents of 52.0 and 51.8 mol%, respectively, as determined by HPLC analysis. Phylogenetic analyses based on 16S rRNA gene sequences clearly indicated that the two isolates formed a distinct genus-level lineage in the family Aeromonadaceae of the class Gammaproteobacteria and was an outgroup with respect to a stable supragenic clade comprising species of the genera Oceanimonas, Oceanisphaera and Zobellella. The phylogenetic data and those from chemotaxonomic, physiological and morphological characterizations support the establishment of a novel species and genus inside the family Aeromonadaceae, for which the name Dongshaea marina gen. nov., sp. nov. is proposed. The type strain is DM2T (=BCRC 81069T=JCM 32096T).
|
| Volume |
69(11)
|
| Pages |
3318-3325
|
| Published |
2019-11-1
|
| DOI |
10.1099/ijsem.0.003080
|
| PMID |
30422104
|
| MeSH |
Aeromonadaceae / classification*
Aeromonadaceae / isolation & purification
Anaerobiosis
Bacterial Typing Techniques
Base Composition
DNA, Bacterial / genetics
Fatty Acids / chemistry
Fermentation
Gases
Geologic Sediments / microbiology*
Glucose / metabolism*
Phospholipids / chemistry
Phylogeny*
RNA, Ribosomal, 16S / genetics
Seawater / microbiology*
Sequence Analysis, DNA
Taiwan
Ubiquinone / chemistry
|
| IF |
2.166
|
| Times Cited |
0
|
| Resource |
| General Microbes |
JCM32096 |
