| RRC ID |
60588
|
| Author |
Sandagdorj B, Hamajima C, Kawahara T, Watanabe J, Tanaka S.
|
| Title |
Characterization of Microbiota that Influence Immunomodulatory Effects of Fermented Brassica rapa L.
|
| Journal |
Microbes Environ
|
| Abstract |
Lactic acid bacteria (LAB) exert beneficial health effects by regulating immune responses. Brassica rapa L., known as Nozawana, is commonly consumed as a lactic acid-fermented food called nozawana-zuke. Few studies have investigated changes in the bacterial community and cytokine production activities during the fermentation of B. rapa L. In order to obtain more detail information, we herein conducted a study on fresh B. rapa L. fermented for 28 d. An amplicon analysis of the 16S rRNA gene revealed that Lactobacillales predominated during fermentation, and the microbiota became less diverse on day 7 or later. Fermented B. rapa L. promoted the production of interferon (IFN)-γ and interleukin (IL)-10 by mouse spleen cells more than non-fermented vegetables. Lactobacillus curvatus was the predominant species during fermentation, followed by L. plantarum and L. brevis. L. sakei was occasionally detected. A correlation analysis showed that IFN-γ concentrations positively correlated with the numbers of L. curvatus and L. plantarum, while those of IL-10 correlated with the numbers of L. sakei in addition to these 2 species. Significantly higher levels of IFN-γ and IL-10 were induced by fermented B. rapa L. when isolated Lactobacillus strains were added as starter cultures. These results suggest that the Lactobacillus species present in fermented B. rapa L. are beneficial for manufacturing vegetables with immunomodulatory effects.
|
| Volume |
34(2)
|
| Pages |
206-214
|
| Published |
2019-6-27
|
| DOI |
10.1264/jsme2.ME19003
|
| PMID |
31167991
|
| PMC |
PMC6594740
|
| MeSH |
Animals
Bacteria / classification
Bacteria / genetics
Bacteria / isolation & purification
Bacteria / metabolism
Biodiversity
Brassica rapa / immunology*
Brassica rapa / microbiology*
Culture Media, Conditioned / pharmacology
Cytokines / metabolism
DNA, Bacterial / genetics
Fermented Foods / microbiology*
Food Microbiology*
Immunologic Factors / pharmacology*
Lactobacillus / classification
Lactobacillus / genetics
Lactobacillus / isolation & purification
Lactobacillus / metabolism
Male
Mice, Inbred C57BL
Microbiota / physiology*
RNA, Ribosomal, 16S / genetics
Species Specificity
Spleen / drug effects
Spleen / metabolism
|
| IF |
2.575
|
| Times Cited |
0
|
| Resource |
| General Microbes |
JCM1059
JCM1096
JCM1149
JCM1157 |
