RRC ID 60683
Author Schalk C, Cognat V, Graindorge S, Vincent T, Voinnet O, Molinier J.
Title Small RNA-mediated repair of UV-induced DNA lesions by the DNA DAMAGE-BINDING PROTEIN 2 and ARGONAUTE 1.
Journal Proc. Natl. Acad. Sci. U.S.A.
Abstract As photosynthetic organisms, plants need to prevent irreversible UV-induced DNA lesions. Through an unbiased, genome-wide approach, we have uncovered a previously unrecognized interplay between Global Genome Repair and small interfering RNAs (siRNAs) in the recognition of DNA photoproducts, prevalently in intergenic regions. Genetic and biochemical approaches indicate that, upon UV irradiation, the DNA DAMAGE-BINDING PROTEIN 2 (DDB2) and ARGONAUTE 1 (AGO1) of Arabidopsis thaliana form a chromatin-bound complex together with 21-nt siRNAs, which likely facilitates recognition of DNA damages in an RNA/DNA complementary strand-specific manner. The biogenesis of photoproduct-associated siRNAs involves the noncanonical, concerted action of RNA POLYMERASE IV, RNA-DEPENDENT RNA POLYMERASE-2, and DICER-LIKE-4. Furthermore, the chromatin association/dissociation of the DDB2-AGO1 complex is under the control of siRNA abundance and DNA damage signaling. These findings reveal unexpected nuclear functions for DCL4 and AGO1, and shed light on the interplay between small RNAs and DNA repair recognition factors at damaged sites.
Volume 114(14)
Pages E2965-E2974
Published 2017-4-4
DOI 10.1073/pnas.1618834114
PII 1618834114
PMID 28325872
PMC PMC5389294
MeSH Arabidopsis / genetics* Arabidopsis / radiation effects Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Argonaute Proteins / genetics Argonaute Proteins / metabolism* Chromatin / metabolism DNA Repair / physiology* DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Gene Expression Regulation, Plant Genome, Plant Mutation Plant Roots / genetics Plant Roots / growth & development Plant Roots / radiation effects Plants, Genetically Modified Pyrimidine Dimers / genetics Pyrimidine Dimers / metabolism RNA, Plant / metabolism* RNA, Small Interfering / genetics RNA, Small Interfering / metabolism Ribonuclease III / genetics Ribonuclease III / metabolism Ultraviolet Rays
IF 9.58
Times Cited 23
Resource
Arabidopsis / Cultured plant cells, genes pst00853