RRC ID 60705
著者 Córdoba-Cañero D, Cognat V, Ariza RR, Roldán Arjona T, Molinier J.
タイトル Dual control of ROS1-mediated active DNA demethylation by DNA damage-binding protein 2 (DDB2).
ジャーナル Plant J
Abstract By controlling gene expression, DNA methylation contributes to key regulatory processes during plant development. Genomic methylation patterns are dynamic and must be properly maintained and/or re-established upon DNA replication and active removal, and therefore require sophisticated control mechanisms. Here we identify direct interplay between the DNA repair factor DNA damage-binding protein 2 (DDB2) and the ROS1-mediated active DNA demethylation pathway in Arabidopsis thaliana. We show that DDB2 forms a complex with ROS1 and AGO4 and that they act at the ROS1 locus to modulate levels of DNA methylation and therefore ROS1 expression. We found that DDB2 represses enzymatic activity of ROS1. DNA demethylation intermediates generated by ROS1 are processed by the DNA 3'-phosphatase ZDP and the apurinic/apyrimidinic endonuclease APE1L, and we also show that DDB2 interacts with both enzymes and stimulates their activities. Taken together, our results indicate that DDB2 acts as a critical regulator of ROS1-mediated active DNA demethylation.
巻・号 92(6)
ページ 1170-1181
公開日 2017-12-1
DOI 10.1111/tpj.13753
PMID 29078035
MeSH Arabidopsis / genetics* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Argonaute Proteins / genetics Argonaute Proteins / metabolism DNA Damage DNA Demethylation DNA Methylation DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Endonucleases / genetics Endonucleases / metabolism Gene Expression Regulation, Plant Nuclear Proteins / genetics Nuclear Proteins / metabolism* Nucleotidases / genetics Nucleotidases / metabolism
IF 6.141
引用数 4
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pst00853