RRC ID 6074
Author Takeo S, Hawley RS, Aigaki T.
Title Calcineurin and its regulation by Sra/RCAN is required for completion of meiosis in Drosophila.
Journal Dev. Biol.
Abstract Ca(2+) signaling pathways play important roles to complete meiosis from metaphase II arrest in vertebrate oocytes. However, less is known about the molecular mechanism of completion of meiosis in Drosophila females. Here, we provide direct evidence that calcineurin, a Ca(2+)/calmodulin (CaM)-dependent phosphatase, is essential for meiotic progression beyond metaphase I in Drosophila oocytes. Oocytes from germline clones lacking CanB2, a calcineurin regulatory subunit B, failed to complete meiosis after egg activation, and laid eggs exhibited a meiotic arrested anaphase I chromosome configuration. Genetic analyses suggest that calcineurin activity is regulated by Sarah (Sra), a family member of regulators of calcineurin (RCANs), through a Sra phosphorylation-dependent mechanism. Our results support a view in which the phosphorylation of Sra not only acts to relieve the inhibitory effects of Sra, but also acts to activate calcineurin, thus explaining the role of RCAN proteins as positive regulators of calcineurin.
Volume 344(2)
Pages 957-67
Published 2010-8-15
DOI 10.1016/j.ydbio.2010.06.011
PII S0012-1606(10)00831-6
PMID 20561515
MeSH Anaphase Animals Calcineurin / genetics Calcineurin / metabolism* Chromosomes / metabolism Drosophila / genetics* Drosophila / metabolism* Female Fertilization / genetics Meiosis Metaphase Oocytes / metabolism Oocytes / physiology Phosphoric Monoester Hydrolases / genetics Phosphoric Monoester Hydrolases / metabolism Phosphorylation Signal Transduction / genetics Signal Transduction / physiology
IF 2.936
Times Cited 27
WOS Category DEVELOPMENTAL BIOLOGY
Resource
Drosophila DGRC#109861 DGRC#109863