| RRC ID |
60898
|
| 著者 |
Hirano Y, Kinugasa Y, Osakada H, Shindo T, Kubota Y, Shibata S, Haraguchi T, Hiraoka Y.
|
| タイトル |
Lem2 and Lnp1 maintain the membrane boundary between the nuclear envelope and endoplasmic reticulum.
|
| ジャーナル |
Commun Biol
|
| Abstract |
The nuclear envelope (NE) continues to the endoplasmic reticulum (ER). Proper partitioning of NE and ER is crucial for cellular activity, but the key factors maintaining the boundary between NE and ER remain to be elucidated. Here we show that the conserved membrane proteins Lem2 and Lnp1 cooperatively play a crucial role in maintaining the NE-ER membrane boundary in fission yeast Schizosaccharomyces pombe. Cells lacking both Lem2 and Lnp1 caused severe growth defects associated with aberrant expansion of the NE/ER membranes, abnormal leakage of nuclear proteins, and abnormal formation of vacuolar-like structures in the nucleus. Overexpression of the ER membrane protein Apq12 rescued the growth defect associated with membrane disorder caused by the loss of Lem2 and Lnp1. Genetic analysis showed that Apq12 had overlapping functions with Lnp1. We propose that a membrane protein network with Lem2 and Lnp1 acts as a critical factor to maintain the NE-ER boundary.
|
| 巻・号 |
3(1)
|
| ページ |
276
|
| 公開日 |
2020-6-1
|
| DOI |
10.1038/s42003-020-0999-9
|
| PII |
10.1038/s42003-020-0999-9
|
| PMID |
32483293
|
| PMC |
PMC7264229
|
| MeSH |
Cell Nucleus / metabolism
DNA-Binding Proteins / genetics*
DNA-Binding Proteins / metabolism
Endoplasmic Reticulum / metabolism*
Membrane Proteins / genetics*
Membrane Proteins / metabolism
Nuclear Envelope / metabolism*
Nuclear Proteins / genetics*
Nuclear Proteins / metabolism
Schizosaccharomyces / genetics*
Schizosaccharomyces / metabolism
Schizosaccharomyces pombe Proteins / genetics*
Schizosaccharomyces pombe Proteins / metabolism
|
| IF |
4.165
|
| 引用数 |
0
|
| リソース情報 |
| 酵母 |
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