RRC ID 61149
Author Numakura Y, Uemura R, Tanaka M, Izawa T, Yamate J, Kuramoto T, Kaneko T, Mashimo T, Yamamoto T, Serikawa T, Kuwamura M.
Title PHF24 is expressed in the inhibitory interneurons in rats.
Journal Exp Anim
Abstract Noda epileptic rat (NER) is a mutant model for epilepsy that exhibits spontaneous generalized tonic-clonic seizure. Epileptogenesis of NER remains to be elucidated; but it is detected an insertion of an endogenous retrovirus sequence in intron 2 of the PHD finger protein 24 (Phf24) gene, encoding Gαi-interacting protein (GINIP). Phf24 is a strong candidate gene for epileptogenesis in NER. PHF24 modulates GABAB signaling through interacting with Gαi protein. To clarify the epileptogenesis of NER, we investigated a distribution of PHF24-expressing cells in the central nerve system (CNS). While broad expression of PHF24 was observed in the CNS, characteristic expression was noted in the periglomerular layer of the olfactory bulb and the lamina II of the spinal cord in the control rats. These cells showed co-expression with calbindin or calretinin, inhibitory interneuron markers. In the olfactory bulb, 15.6% and 41.2% of PHF24-positive neurons co-expressed calbindin and calretinin, respectively. Immunoelectron microscopy revealed that PHF24 was located in the presynaptic terminals, synaptic membranes and cytoplasmic matrix of neuronal soma. Our data suggested PHF24 is expressed in the inhibitory interneurons and may play important roles in modulation of the GABAB signaling.
Volume 70(1)
Pages 137-143
Published 2021-2-6
DOI 10.1538/expanim.20-0105
PMID 33115988
PMC PMC7887615
MeSH Animals Calbindin 2 / metabolism Calbindins / metabolism Central Nervous System / cytology Central Nervous System / metabolism Disease Models, Animal Gene Expression* Genetic Association Studies* Homeodomain Proteins / genetics* Homeodomain Proteins / metabolism* Immunohistochemistry Interneurons / metabolism* Intracellular Signaling Peptides and Proteins / genetics Intracellular Signaling Peptides and Proteins / metabolism Olfactory Bulb / metabolism Rats, Inbred F344 Seizures / genetics* Seizures / metabolism* Signal Transduction / genetics Spinal Cord / metabolism gamma-Aminobutyric Acid / metabolism
IF 1.574
Rats NER/Kyo (StrainID=26) F344/Stm (StrainID=266)