RRC ID 61191
著者 Tateno H, Onuma Y, Ito Y, Hiemori K, Aiki Y, Shimizu M, Higuchi K, Fukuda M, Warashina M, Honda S, Asashima M, Hirabayashi J.
タイトル A medium hyperglycosylated podocalyxin enables noninvasive and quantitative detection of tumorigenic human pluripotent stem cells.
ジャーナル Sci Rep
Abstract While human pluripotent stem cells are attractive sources for cell-replacement therapies, a major concern remains regarding their tumorigenic potential. Thus, safety assessment of human pluripotent stem cell-based products in terms of tumorigenicity is critical. Previously we have identified a pluripotent stem cell-specific lectin probe rBC2LCN recognizing hyperglycosylated podocalyxin as a cell surface ligand. Here we demonstrate that hyperglycosylated podocalyxin is secreted from human pluripotent stem cells into cell culture supernatants. We establish a sandwich assay system, named the GlycoStem test, targeting the soluble hyperglycosylated podocalyxin using rBC2LCN. The GlycoStem test is sufficiently sensitive and quantitative to detect residual human pluripotent stem cells. This work provides a proof of concept for the noninvasive and quantitative detection of tumorigenic human pluripotent stem cells using cell culture supernatants. The developed method should increase the safety of human pluripotent stem cell-based cell therapies.
巻・号 4
ページ 4069
公開日 2014-2-12
DOI 10.1038/srep04069
PII srep04069
PMID 24518842
PMC PMC3921628
MeSH Biotin / chemistry Biotinylation Burkholderia cenocepacia / metabolism Cell Differentiation Cell Transformation, Neoplastic* Cells, Cultured Coculture Techniques Flow Cytometry Glycosylation HEK293 Cells Humans Lectins / chemistry Lectins / genetics Lectins / metabolism Ligands Pluripotent Stem Cells / cytology* Pluripotent Stem Cells / metabolism Protein Array Analysis / methods Sialoglycoproteins / chemistry Sialoglycoproteins / metabolism*
IF 3.998
リソース情報
ヒト・動物細胞 201B7(HPS0063) 253G1(HPS0002)