| RRC ID |
6132
|
| 著者 |
Fujii T, Daimon T, Uchino K, Banno Y, Katsuma S, Sezutsu H, Tamura T, Shimada T.
|
| タイトル |
Transgenic analysis of the BmBLOS2 gene that governs the translucency of the larval integument of the silkworm, Bombyx mori.
|
| ジャーナル |
Insect Mol Biol
|
| Abstract |
The larval integument of the silkworm, Bombyx mori, is opaque because urate granules accumulate in the epidermis. Although the biosynthetic pathway of uric acid is well studied, little is known about how uric acid accumulates as urate granules in epidermal cells. In the distinct oily (od) mutant silkworm, the larval integument is translucent because of the inability to construct urate granules. Recently, we have found that the od mutant has a genomic deletion in the B. mori homologue of the human biogenesis of lysosome-related organelles complex1, subunit 2 (BLOS2) gene (BmBLOS2). Here, we performed a molecular and functional characterization of BmBLOS2. Northern blot analysis showed that BmBLOS2 was ubiquitously expressed in various tissues. We analysed the structure of a newly isolated mutant (od(B) ) allelic to od and found a premature stop codon in the coding sequence of BmBLOS2 in this new mutation. Moreover, the translucent phenotype was rescued by the germ-line transformation of the wild-type BmBLOS2 allele into the od mutant. Our results suggest that BmBLOS2 is responsible for the od mutant phenotype and plays a crucial role in biogenesis of urate granules in the larval epidermis of the silkworm. The relationships amongst Hermansky-Pudlak syndrome (HPS) genes in mammals, granule group genes in Drosophila and translucent mutant genes in B. mori are discussed.
|
| 巻・号 |
19(5)
|
| ページ |
659-67
|
| 公開日 |
2010-10-1
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| DOI |
10.1111/j.1365-2583.2010.01020.x
|
| PII |
IMB1020
|
| 解説 |
od(d油）の原因遺伝子はBmBlos2である。BmBlos2はヒトのヘルマンスキーパドラック症候群の原因遺伝子の一つであるBlos2のカイコホモログである。od系統では、BmBlos2のエクソン１～３が欠失している。この欠失には、BmBlos2のエクソン１の上流とイントロン３に共通して存在するトランスポゾンBm1の関与が推定されている。o05系統(odb/odb)では、エクソン４に中途終始コドンを生じさせる点突然変異が存在する。
|
| PMID |
20546041
|
| MeSH |
Alleles
Animals
Animals, Genetically Modified
Blotting, Northern
Bombyx / anatomy & histology*
Bombyx / genetics*
Bombyx / growth & development
Epidermis / metabolism
Female
Gene Expression Profiling
Genes, Insect
Larva / genetics
Male
Molecular Sequence Data
Mutation
Phenotype
Pigmentation
Sequence Analysis, DNA
Transgenes
Uric Acid / metabolism
|
| IF |
2.533
|
| 引用数 |
34
|
|
WOS 分野
|
ENTOMOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| カイコ |
o05 |
