論文 - 詳細
| RRC ID | 61363 |
|---|---|
| 著者 | Toma-Fukai S, Hibi R, Naganuma T, Sakai M, Saijo S, Shimizu N, Matsumoto M, Shimizu T. |
| タイトル | Crystal structure of GCN5 PCAF N-terminal domain reveals atypical ubiquitin ligase structure. |
| ジャーナル | J Biol Chem |
| Abstract |
General control nonderepressible 5 (GCN5, also known as Kat2a) and p300/CBP-associated factor (PCAF, also known as Kat2b) are two homologous acetyltransferases. Both proteins share similar domain architecture consisting of a PCAF N-terminal (PCAF_N) domain, acetyltransferase domain, and a bromodomain. PCAF also acts as a ubiquitin E3 ligase whose activity is attributable to the PCAF_N domain, but its structural aspects are largely unknown. Here, we demonstrated that GCN5 exhibited ubiquitination activity in a similar manner to PCAF and its activity was supported by the ubiquitin-conjugating enzyme UbcH5. Moreover, we determined the crystal structure of the PCAF_N domain at 1.8 Å resolution and found that PCAF_N domain folds into a helical structure with a characteristic binuclear zinc region, which was not predicted from sequence analyses. The zinc region is distinct from known E3 ligase structures, suggesting this region may form a new class of E3 ligase. Our biochemical and structural study provides new insight into not only the functional significance of GCN5 but also into ubiquitin biology. |
| 巻・号 | 295(43) |
| ページ | 14630-14639 |
| 公開日 | 2020-10-23 |
| DOI | 10.1074/jbc.RA120.013431 |
| PII | S0021-9258(17)49341-0 |
| PMID | 32820047 |
| PMC | PMC7586209 |
| MeSH | Animals Crystallography, X-Ray Humans Mice Models, Molecular Protein Conformation Protein Domains Ubiquitin-Protein Ligases / chemistry* Ubiquitin-Protein Ligases / metabolism Ubiquitination p300-CBP Transcription Factors / chemistry* p300-CBP Transcription Factors / metabolism |
| IF | 4.238 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | Genome Network Project Human cDNA Clone IRAK072A12 (HGY028812) IRAK133C02 (HGY053250) |