RRC ID 61555
著者 Murayama A, Ohmori K, Fujimura A, Minami H, Yasuzawa-Tanaka K, Kuroda T, Oie S, Daitoku H, Okuwaki M, Nagata K, Fukamizu A, Kimura K, Shimizu T, Yanagisawa J.
タイトル Epigenetic control of rDNA loci in response to intracellular energy status.
ジャーナル Cell
Abstract Intracellular energy balance is important for cell survival. In eukaryotic cells, the most energy-consuming process is ribosome biosynthesis, which adapts to changes in intracellular energy status. However, the mechanism that links energy status and ribosome biosynthesis is largely unknown. Here, we describe eNoSC, a protein complex that senses energy status and controls rRNA transcription. eNoSC contains Nucleomethylin, which binds histone H3 dimethylated Lys9 in the rDNA locus, in a complex with SIRT1 and SUV39H1. Both SIRT1 and SUV39H1 are required for energy-dependent transcriptional repression, suggesting that a change in the NAD(+)/NADH ratio induced by reduction of energy status could activate SIRT1, leading to deacetylation of histone H3 and dimethylation at Lys9 by SUV39H1, thus establishing silent chromatin in the rDNA locus. Furthermore, eNoSC promotes restoration of energy balance by limiting rRNA transcription, thus protecting cells from energy deprivation-dependent apoptosis. These findings provide key insight into the mechanisms of energy homeostasis in cells.
巻・号 133(4)
ページ 627-39
公開日 2008-5-16
DOI 10.1016/j.cell.2008.03.030
PII S0092-8674(08)00459-5
PMID 18485871
MeSH Cell Death Cell Line Cell Nucleolus / metabolism DNA, Ribosomal / genetics* Energy Metabolism* Gene Silencing* Glucose / metabolism Histone Methyltransferases Histone-Lysine N-Methyltransferase / chemistry Histone-Lysine N-Methyltransferase / metabolism Histones / metabolism Humans Methyltransferases / chemistry Methyltransferases / metabolism Models, Molecular Molecular Sequence Data Multiprotein Complexes / chemistry Multiprotein Complexes / metabolism NAD / metabolism Nuclear Proteins / chemistry Nuclear Proteins / metabolism Protein Methyltransferases Protein Structure, Tertiary RNA-Binding Proteins Repressor Proteins / metabolism Sirtuin 1 Sirtuins / metabolism Transcription, Genetic*
IF 38.637
リソース情報
ヒト・動物細胞 NB1RGB(RCB0222)