論文 - 詳細
| RRC ID | 61564 |
|---|---|
| 著者 | Ito S, Tanaka Y, Nishio N, Thanasegaran S, Isobe K. |
| タイトル | Establishment of self-renewable GM-CSF-dependent immature macrophages in vitro from murine bone marrow. |
| ジャーナル | PLoS One |
| Abstract |
Macrophages play a key role in the innate immune system. Macrophages are thought to originate from hematopoietic precursors or the yolk sac. Here, we describe the in vitro establishment of self-renewable GM-CSF-dependent immature macrophages (GM-IMs) from murine bone marrow (BM). GM-IMs grow continuously in vitro in conditioned medium containing GM-CSF. The immunophenotype of GM-IMs is F4/80(high) CD11b(high) CD11c(low) Ly6C(low). By comparing gene expression in GM-IMs and BM dendritic cells, we found that GM-IMs expressed lower levels of chemokines, cytokines and their receptors. GM-IMs are round in shape, attach loosely to non-coated culture dishes and have a marked phagocytic capacity. These results indicate that GM-IMs are macrophage precursor cells. Following stimulation with LPS, monocyte-like GM-IMs converted to flat macrophage-like cells that tightly adhered to non-coated culture dishes and produced pro-inflammatory cytokines TNFα, IL-6 and IL-1β. These results indicated that GM-IMs differentiated to M1 pro-inflammatory macrophages. This was confirmed by stimulation of GM-IMs with IFNγ, an inducer of M1 markers. GM-IMs showed enhanced expression of M2 macrophage markers such as Arg1 and Retnla following stimulation by Th2 cytokines IL-4 and IL-13. When GM-IMs were injected into mice at sites of wounding, wound repair was enhanced. These results indicate that GM-IMs can differentiate to M2 macrophages. When GM-IMs were injected into clodronate-treated mice, they induced resident macrophage proliferation by producing M-CSF. In conclusion we have established self-renewable GM-CSF-dependent immature macrophages in vitro from murine BM, which differentiate to M1 or M2 macrophages. |
| 巻・号 | 8(10) |
| ページ | e76943 |
| 公開日 | 2013-1-1 |
| DOI | 10.1371/journal.pone.0076943 |
| PII | PONE-D-13-27019 |
| PMID | 24124601 |
| PMC | PMC3790761 |
| MeSH | Animals Bone Marrow Cells / cytology Bone Marrow Cells / drug effects Bone Marrow Cells / metabolism* Cell Differentiation Cell Lineage Cell Proliferation / drug effects Female Gene Expression Profiling Granulocyte-Macrophage Colony-Stimulating Factor / metabolism* Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology Hematopoietic Stem Cell Transplantation Immunophenotyping Macrophages / cytology Macrophages / drug effects Macrophages / metabolism* Mice Myeloid Progenitor Cells / cytology Myeloid Progenitor Cells / drug effects Myeloid Progenitor Cells / metabolism Phenotype Primary Cell Culture |
| IF | 2.74 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |