Reference - Detail
| RRC ID | 61645 |
|---|---|
| Author | Terada M, Shimizu A, Sato N, Miyakaze SI, Katayama H, Kurokawa-Seo M. |
| Title | Fibroblast growth factor receptor 3 lacking the Ig IIIb and transmembrane domains secreted from human squamous cell carcinoma DJM-1 binds to FGFs. |
| Journal | Mol Cell Biol Res Commun |
| Abstract |
The fibroblast growth factor receptors (FGFRs) are a family of transmembrane tyrosine kinases that play a key role in cell growth and tumorigenesis in response to FGFs. FGFR complexity is increased by the existence of additional isoforms generated by alternative mRNA splicing. We identified that the transcript FGFR3DeltaTM, an alternatively spliced isoform of FGFR3 lacking exons encoding the C-terminal half of Ig III (IIIb) and transmembrane domains, is expressed in the human squamous carcinoma cell line DJM-1. To determine whether FGFR3DeltaTM has the potential to be secreted, we analyzed the protein expression in CHOK1 cells transfected with FGFR3DeltaTM cDNA and DJM-1 cells. Western blot analysis revealed that FGFR3DeltaTM protein was secreted, N-glycosylated, and dimerized by an intermolecular disulfide bond. Cross-linking experiments showed that FGF1 and FGF2 were able to bind to FGFR3DeltaTM, suggesting that the loss of the Ig IIIb domain may confer upon FGFR3DeltaTM the ability to bind to FGF2. |
| Volume | 4(6) |
| Pages | 365-73 |
| Published | 2001-11-1 |
| DOI | 10.1006/mcbr.2001.0306 |
| PII | S152247240190306X |
| PMID | 11703096 |
| MeSH | Alternative Splicing Amino Acid Sequence Animals Base Sequence Blotting, Western CHO Cells Carcinoma, Squamous Cell / genetics Carcinoma, Squamous Cell / metabolism Cell Membrane / metabolism Cells, Cultured Cloning, Molecular Cricetinae Cross-Linking Reagents / pharmacology DNA, Complementary / metabolism Disulfides Exons Glycoside Hydrolases / pharmacology Glycosylation Heparin / metabolism Humans Models, Biological Molecular Sequence Data Precipitin Tests Protein Binding Protein Isoforms Protein Structure, Tertiary Protein-Tyrosine Kinases* RNA / metabolism Receptor, Fibroblast Growth Factor, Type 3 Receptors, Fibroblast Growth Factor / chemistry* Receptors, Fibroblast Growth Factor / genetics Receptors, Fibroblast Growth Factor / metabolism* Recombinant Proteins / metabolism Reverse Transcriptase Polymerase Chain Reaction Sepharose / pharmacology Transfection Tumor Cells, Cultured |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 18 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | HBL-100(RCB0460) CHO-K1(RCB0285) |