論文 - 詳細
| RRC ID | 61938 |
|---|---|
| 著者 | Yamamoto A, Miyazaki T, Kadono Y, Takayanagi H, Miura T, Nishina H, Katada T, Wakabayashi K, Oda H, Nakamura K, Tanaka S. |
| タイトル | Possible involvement of IkappaB kinase 2 and MKK7 in osteoclastogenesis induced by receptor activator of nuclear factor kappaB ligand. |
| ジャーナル | J Bone Miner Res |
| Abstract |
Recent studies have revealed the essential role of the receptor activator of nuclear factor kappaB (NF-kappaB) ligand (RANKL) in osteoclast differentiation and activation. Adenovirus vector could efficiently transduce genes into RAW264.7 cells, which differentiate into osteoclast-like multinucleated cells in the presence of RANKL. The role of NF-kappaB and c-jun N-terminal kinase (JNK) activation in RANKL-induced osteoclast differentiation was investigated using an adenovirus vector carrying the dominant negative 1kappaB kinase 2 gene (AxIKK2DN) or dominant negative MKK7 gene (AxMKK7DN). IKK2DN and MKK7DN overexpression in RAW cells specifically suppressed the NF-kappaB activation and JNK activation in response to RANKL, respectively, without affecting other signaling pathways. Either inhibition of NF-kappaB or JNK pathways dose-dependently inhibited osteoclast formation induced by RANKL. These results suggest that both NF-kappaB and JNK activation are independently required for osteoclast differentiation. |
| 巻・号 | 17(4) |
| ページ | 612-21 |
| 公開日 | 2002-4-1 |
| DOI | 10.1359/jbmr.2002.17.4.612 |
| PMID | 11918218 |
| MeSH | Adenoviridae / genetics Animals Carrier Proteins / metabolism* Cell Differentiation / genetics Cells, Cultured Enzyme Activation Genes, Dominant I-kappa B Kinase JNK Mitogen-Activated Protein Kinases MAP Kinase Kinase 7 Male Membrane Glycoproteins / metabolism* Mice Mice, Inbred Strains Mitogen-Activated Protein Kinase Kinases / genetics Mitogen-Activated Protein Kinase Kinases / metabolism* Mitogen-Activated Protein Kinases / metabolism Osteoclasts / physiology* Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / metabolism* RANK Ligand Receptor Activator of Nuclear Factor-kappa B Stem Cells / physiology |
| IF | 5.854 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |