RRC ID 62041
Author Higuchi T, Nishikawa J, Inoue H.
Title Sucrose induces vesicle accumulation and autophagy.
Journal J Cell Biochem
Abstract It has been shown that the treatment of mammalian cells with sucrose leads to vacuole accumulation associated with lysosomes and upregulation of lysosomal enzyme expression and activity. Autophagy is an evolutionarily conserved homeostatic process by which cells deliver cytoplasmic material for degradation into lysosomes, thus it is probable that sucrose affects the autophagic activity. The role of sucrose in autophagy is unknown; however, another disaccharide, trehalose has been shown to induce autophagy. In the current study, we used mouse embryonic fibroblasts to investigate whether sucrose induces autophagy and whether vesicle formation is associated with autophagy. The results showed that sucrose induces autophagy while being accumulated within the endosomes/lysosomes. These vesicles were swollen and packed within the cytoplasm. Furthermore, trehalose and the trisaccharide raffinose, which are not hydrolyzed in mammalian cells, increased the rate of vesicles accumulation and LC3-II level (a protein marker of autophagy). However, fructose and maltose did not show the same effects. The correlation between the two processes, vesicle accumulation and autophagy induction, was confirmed by treatment of cells with sucrose plus invertase, or maltose plus acarbose-the α-glucosidase inhibitor-and by sucrose deprivation. Results also showed that vesicle accumulation was not affected by autophagy inhibition. Therefore, the data suggest that sucrose-induced autophagy through accumulation of sucrose-containing vesicles is caused by the absence of hydrolysis enzymes.
Volume 116(4)
Pages 609-17
Published 2015-4-1
DOI 10.1002/jcb.25012
PMID 25389129
MeSH Animals Autophagy / drug effects Cells, Cultured Embryo, Mammalian / cytology Endosomes / chemistry Endosomes / metabolism* Fibroblasts / cytology Fibroblasts / drug effects* Hydrolysis Lysosomes / chemistry Lysosomes / metabolism* Mice Microtubule-Associated Proteins / metabolism Raffinose / pharmacology Sucrose / pharmacokinetics Sucrose / pharmacology* Trehalose / pharmacology
IF 4.237
Human and Animal Cells Atg5^(-/-)MEF(RCB2711)