論文 - 詳細
| RRC ID | 6268 |
|---|---|
| 著者 | Miyasaka N, Knaut H, Yoshihara Y. |
| タイトル | Cxcl12/Cxcr4 chemokine signaling is required for placode assembly and sensory axon pathfinding in the zebrafish olfactory system. |
| ジャーナル | Development |
| Abstract |
Positioning neurons in the right places and wiring axons to the appropriate targets are essential events for establishment of neural circuits. In the zebrafish olfactory system, precursors of olfactory sensory neurons (OSNs) assemble into a compact cluster to form the olfactory placode. Subsequently, OSNs differentiate and extend their axons to the presumptive olfactory bulb with high precision. In this study, we aim to elucidate the molecular mechanism underlying these two developmental processes. cxcr4b, encoding a chemokine receptor, is expressed in the migrating olfactory placodal precursors, and cxcl12a (SDF-1a), encoding a ligand for Cxcr4b, is expressed in the abutting anterior neural plate. The expression of cxcr4b persists in the olfactory placode at the initial phase of OSN axon pathfinding. At this time, cxcl12a is expressed along the placode-telencephalon border and at the anterior tip of the telencephalon, prefiguring the route and target of OSN axons, respectively. Interfering with Cxcl12a/Cxcr4b signaling perturbs the assembly of the olfactory placode, resulting in the appearance of ventrally displaced olfactory neurons. Moreover, OSN axons frequently fail to exit the olfactory placode and accumulate near the placode-telencephalon border in the absence of Cxcr4b-mediated signaling. These data indicate that chemokine signaling contributes to both the olfactory placode assembly and the OSN axon pathfinding in zebrafish. |
| 巻・号 | 134(13) |
| ページ | 2459-68 |
| 公開日 | 2007-7-1 |
| DOI | 10.1242/dev.001958 |
| PII | dev.001958 |
| PMID | 17537794 |
| MeSH | Animals Animals, Genetically Modified Axons / metabolism* Chemokine CXCL12 Chemokines, CXC / genetics Chemokines, CXC / metabolism* Gene Expression Regulation, Developmental Mutation / genetics Neurons, Afferent / metabolism* Olfactory Pathways / cytology Olfactory Pathways / embryology Olfactory Pathways / metabolism* Phenotype Receptors, CXCR4 / genetics Receptors, CXCR4 / metabolism* Signal Transduction* Zebrafish / embryology Zebrafish / genetics Zebrafish / metabolism* Zebrafish Proteins / genetics Zebrafish Proteins / metabolism |
| IF | 5.611 |
| 引用数 | 56 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | rw032 (Tg(OMP2k :gap-YFP)) |