| RRC ID |
62723
|
| 著者 |
Aiyama T, Orimo T, Yokoo H, Ohata T, Hatanaka KC, Hatanaka Y, Fukai M, Kamiyama T, Taketomi A.
|
| タイトル |
Adenomatous polyposis coli-binding protein end-binding 1 promotes hepatocellular carcinoma growth and metastasis.
|
| ジャーナル |
PLoS One
|
| Abstract |
This study was performed to determine the clinical significance of adenomatous polyposis coli (APC)-binding protein end-binding 1 (EB1) in hepatocellular carcinoma (HCC) and to characterize its biochemical role in comparison with previous reports. We performed immunohistochemical staining to detect EB1 expression in tissues from 235 patients with HCC and investigated its correlations with clinicopathological features and prognosis. We also investigated the roles of EB1 in cell proliferation, migration, and tumorigenesis in vitro and in vivo by siRNA- and CRISPR/Cas9-mediated modulation of EB1 expression in human HCC cell lines. The results showed that EB1 expression was significantly correlated with several important factors associated with tumor malignancy, including histological differentiation, portal vein invasion status, and intrahepatic metastasis. Patients with high EB1 expression in HCC tissue had poorer overall survival and higher recurrence rates than patients with low EB1 expression. EB1 knockdown and knockout in HCC cells reduced cell proliferation, migration, and invasion in vitro and inhibited tumor growth in vivo. Further, genes encoding Dlk1, HAMP, and SLCO1B3 that were differentially expressed in association with EB1 were identified using RNA microarray analysis. In conclusion, elevated expression of EB1 promotes tumor growth and metastasis of HCC. EB1 may serve as a new biomarker for HCC, and genes coexpressed with EB1 may represent potential targets for therapy.
|
| 巻・号 |
15(9)
|
| ページ |
e0239462
|
| 公開日 |
2020-1-1
|
| DOI |
10.1371/journal.pone.0239462
|
| PII |
PONE-D-20-12629
|
| PMID |
32956413
|
| PMC |
PMC7505586
|
| MeSH |
Adult
Aged
Animals
Carcinoma, Hepatocellular / complications
Carcinoma, Hepatocellular / genetics
Carcinoma, Hepatocellular / pathology*
Cell Differentiation
Cell Line, Tumor
Cell Proliferation
Female
Gene Expression Regulation, Neoplastic
Gene Knockout Techniques
Genes, APC
Hepatitis, Viral, Human / complications
Heterografts
Humans
Kaplan-Meier Estimate
Liver Neoplasms / complications
Liver Neoplasms / genetics
Liver Neoplasms / pathology*
Male
Mice, Inbred BALB C
Mice, Nude
Microtubule-Associated Proteins / antagonists & inhibitors
Microtubule-Associated Proteins / genetics
Microtubule-Associated Proteins / physiology*
Middle Aged
Neoplasm Invasiveness
Neoplasm Metastasis
Neoplasm Proteins / antagonists & inhibitors
Neoplasm Proteins / genetics
Neoplasm Proteins / physiology*
Portal Vein / pathology
Prognosis
RNA Interference
RNA, Messenger / biosynthesis
RNA, Messenger / genetics
RNA, Neoplasm / biosynthesis
RNA, Neoplasm / genetics
RNA, Small Interfering / genetics
Recombinant Proteins / metabolism
Recurrence
Survival Rate
Tissue Array Analysis
|
| IF |
2.74
|
| リソース情報 |
| ヒト・動物細胞 |
Li-7(RCB1941) |
