RRC ID 62797
著者 Watanabe S, Usui-Kawanishi F, Karasawa T, Kimura H, Kamata R, Komada T, Inoue Y, Mise N, Kasahara T, Takahashi M.
タイトル Glucose regulates hypoxia-induced NLRP3 inflammasome activation in macrophages.
ジャーナル J Cell Physiol
Abstract Although the intimate linkage between hypoxia and inflammation is well known, the mechanism underlying this linkage has not been fully understood. Nucleotide-binding oligomerization domain-like receptor (NLR) family pyrin domain containing 3 (NLRP3) inflammasome is an intracellular multiprotein complex that regulates interleukin-1β (IL-1β) secretion and pyroptosis, and is implicated in the pathogenesis of sterile inflammatory diseases. Here, we investigated the regulatory mechanism of NLRP3 inflammasome activation in response to hypoxia in macrophages. Severe hypoxia (0.1% O2 ) induced the processing of pro-IL-1β, pro-caspase-1, and gasdermin D, as well as the release of IL-1β and lactate dehydrogenase in lipopolysaccharide (LPS)-primed murine macrophages, indicating that hypoxia induces NLRP3 inflammasome-driven inflammation and pyroptosis. NLRP3 deficiency and a specific caspase-1 blockade inhibited hypoxia-induced IL-1β release. Hypoxia-induced IL-1β release and cell death were augmented under glucose deprivation, and an addition of glucose in the media negatively regulated hypoxia-induced IL-1β release. Under hypoxia and glucose deprivation, hypoxia-induced glycolysis was not driven and subsequently, the intracellular adenosine triphosphates (ATPs) were depleted. Atomic absorption spectrometry analysis showed a reduction of intracellular K+ concentrations, indicating the K+ efflux occurring under hypoxia and glucose deprivation. Furthermore, hypoxia and glucose deprivation-induced IL-1β release was significantly prevented by inhibition of K+ efflux and KATP channel blockers. In vivo experiments further revealed that IL-1β production was increased in LPS-primed mice exposed to hypoxia (9.5% O2 ), which was prevented by a deficiency of NLRP3, an apoptosis-associated speck-like protein containing a caspase recruitment domain, and caspase-1. Our results demonstrate that NLRP3 inflammasome can sense intracellular energy crisis as a danger signal induced by hypoxia and glucose deprivation, and provide new insights into the mechanism underlying hypoxia-induced inflammation.
巻・号 235(10)
ページ 7554-7566
公開日 2020-10-1
DOI 10.1002/jcp.29659
PMID 32115713
MeSH Adenosine Triphosphate / metabolism Animals Apoptosis / drug effects Caspase 1 / metabolism Cell Death / drug effects Cells, Cultured Glucose / metabolism* Hypoxia / metabolism* Inflammasomes / metabolism* Inflammation / metabolism Interleukin-1beta / metabolism Lipopolysaccharides / pharmacology Macrophages / drug effects Macrophages / metabolism* Male Mice Mice, Inbred C57BL NLR Family, Pyrin Domain-Containing 3 Protein / metabolism* Potassium / metabolism Signal Transduction / drug effects Signal Transduction / physiology
IF 5.546
リソース情報
ヒト・動物細胞 J774.1(RCB0434)