| RRC ID |
6284
|
| 著者 |
Kim TW, Guan S, Sun Y, Deng Z, Tang W, Shang JX, Sun Y, Burlingame AL, Wang ZY.
|
| タイトル |
Brassinosteroid signal transduction from cell-surface receptor kinases to nuclear transcription factors.
|
| ジャーナル |
Nat Cell Biol
|
| Abstract |
Brassinosteroid (BR) regulates gene expression and plant development through a receptor kinase-mediated signal transduction pathway. Despite the identification of many components of this pathway, it remains unclear how the BR signal is transduced from the cell surface to the nucleus. Here we describe a complete BR signalling pathway by elucidating key missing steps. We show that phosphorylation of BSK1 (BR-signalling kinase 1) by the BR receptor kinase BRI1 (BR-insensitive 1) promotes BSK1 binding to the BSU1 (BRI1 suppressor 1) phosphatase, and BSU1 inactivates the GSK3-like kinase BIN2 (BR-insensitive 2) by dephosphorylating a conserved phospho-tyrosine residue (pTyr 200). Mutations that affect phosphorylation/dephosphorylation of BIN2 pTyr200 (bin2-1, bin2-Y200F and quadruple loss-of-function of BSU1-related phosphatases) support an essential role for BSU1-mediated BIN2 dephosphorylation in BR-dependent plant growth. These results demonstrate direct sequential BR activation of BRI1, BSK1 and BSU1, and inactivation of BIN2, leading to accumulation of unphosphorylated BZR (brassinazole resistant) transcription factors in the nucleus. This study establishes a fully connected BR signalling pathway and provides new insights into the mechanism of GSK3 regulation.
|
| 巻・号 |
11(10)
|
| ページ |
1254-60
|
| 公開日 |
2009-10-1
|
| DOI |
10.1038/ncb1970
|
| PII |
ncb1970
|
| PMID |
19734888
|
| PMC |
PMC2910619
|
| MeSH |
Amino Acid Sequence
Binding Sites / genetics
Brassinosteroids
Cell Nucleus / genetics
Cell Nucleus / metabolism*
Cholestanols / pharmacology
Gene Expression Regulation, Plant
Genes, Plant
Glutathione Transferase / metabolism
Glycogen Synthase Kinase 3 / chemistry
Glycogen Synthase Kinase 3 / genetics
Glycogen Synthase Kinase 3 / metabolism
Mutation
Nuclear Proteins / chemistry
Nuclear Proteins / genetics
Nuclear Proteins / metabolism
Phosphorylation / drug effects
Phytosterols / metabolism
Phytosterols / pharmacology
Plant Growth Regulators / pharmacology
Plant Proteins / chemistry
Plant Proteins / genetics
Plant Proteins / metabolism*
Plants, Genetically Modified
Protein Binding
Receptors, Cell Surface / genetics
Receptors, Cell Surface / metabolism*
Recombinant Fusion Proteins / chemistry
Recombinant Fusion Proteins / metabolism
Signal Transduction / genetics
Signal Transduction / physiology*
Steroids, Heterocyclic / metabolism*
Steroids, Heterocyclic / pharmacology
Subcellular Fractions / metabolism
|
| IF |
20.042
|
| 引用数 |
329
|
|
WOS 分野
|
CELL BIOLOGY
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
pda09652 |
