RRC ID 62920
Author Huang Y, Xiao D, Huang S, Zhuang J, Zheng X, Chang Y, Yin D.
Title Circular RNA YAP1 attenuates osteoporosis through up-regulation of YAP1 and activation of Wnt/β-catenin pathway.
Journal Biomed Pharmacother
Abstract BACKGROUND:Osteoporosis is a systemic bone disease resulting from decreased bone mass and bone microstructure degeneration. Yes-associated protein 1 (YAP1) belongs to YAP family and plays a significant part in controlling bone quality.
AIM OF THE STUDY:Present study aimed to study the function and up-stream mechanism of YAP1 in the differentiation of BMSCs (bone marrow stromal cells) and MC3T3-E1.
METHODS:ALP staining, alizarin red staining and western blot analysis of osteogenic biomarkers determined osteogenic differentiation in BMSCs and MC3T3-E1. Mechanistic assays including luciferase reporter assay, RIP assay and RNA pull down assay disclosed the interplays between RNAs.
RESULTS:YAP1 promoted osteogenic differentiation of BMSCs and MC3T3-E1. Circ_0024097 originated from YAP1 sponged miR-376b-3p to elevate YAP1 expression in BMSCs and MC3T3-E1. Further, YAP1 mediated circ_0024097- promoted effects on osteogenic differentiation. Moreover, circ_0024097 activated Wnt/β-catenin pathway to facilitate osteogenic differentiation.
CONCLUSION:It was firstly uncovered in present study that circ_0024097 attenuated osteoporosis through promoting osteogenic differentiation via miR-376b-3p/YAP1 axis and Wnt/β-catenin pathway.
Volume 129
Pages 110365
Published 2020-9-1
DOI 10.1016/j.biopha.2020.110365
PII S0753-3322(20)30558-8
PMID 32768931
MeSH 3T3 Cells Adaptor Proteins, Signal Transducing / genetics Adaptor Proteins, Signal Transducing / metabolism* Animals Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Cell Differentiation* Mesenchymal Stem Cells / metabolism* Mesenchymal Stem Cells / pathology Mice MicroRNAs / genetics MicroRNAs / metabolism Osteoblasts / metabolism* Osteoblasts / pathology Osteogenesis* Osteoporosis / genetics Osteoporosis / metabolism* Osteoporosis / pathology RNA, Circular / genetics RNA, Circular / metabolism Up-Regulation Wnt Signaling Pathway* YAP-Signaling Proteins
IF 4.545
Human and Animal Cells MC3T3-E1(RCB1126)