論文 - 詳細
| RRC ID | 62920 |
|---|---|
| 著者 | Huang Y, Xiao D, Huang S, Zhuang J, Zheng X, Chang Y, Yin D. |
| タイトル | Circular RNA YAP1 attenuates osteoporosis through up-regulation of YAP1 and activation of Wnt/β-catenin pathway. |
| ジャーナル | Biomed Pharmacother |
| Abstract |
BACKGROUND:Osteoporosis is a systemic bone disease resulting from decreased bone mass and bone microstructure degeneration. Yes-associated protein 1 (YAP1) belongs to YAP family and plays a significant part in controlling bone quality. AIM OF THE STUDY:Present study aimed to study the function and up-stream mechanism of YAP1 in the differentiation of BMSCs (bone marrow stromal cells) and MC3T3-E1. METHODS:ALP staining, alizarin red staining and western blot analysis of osteogenic biomarkers determined osteogenic differentiation in BMSCs and MC3T3-E1. Mechanistic assays including luciferase reporter assay, RIP assay and RNA pull down assay disclosed the interplays between RNAs. RESULTS:YAP1 promoted osteogenic differentiation of BMSCs and MC3T3-E1. Circ_0024097 originated from YAP1 sponged miR-376b-3p to elevate YAP1 expression in BMSCs and MC3T3-E1. Further, YAP1 mediated circ_0024097- promoted effects on osteogenic differentiation. Moreover, circ_0024097 activated Wnt/β-catenin pathway to facilitate osteogenic differentiation. CONCLUSION:It was firstly uncovered in present study that circ_0024097 attenuated osteoporosis through promoting osteogenic differentiation via miR-376b-3p/YAP1 axis and Wnt/β-catenin pathway. |
| 巻・号 | 129 |
| ページ | 110365 |
| 公開日 | 2020-9-1 |
| DOI | 10.1016/j.biopha.2020.110365 |
| PII | S0753-3322(20)30558-8 |
| PMID | 32768931 |
| MeSH | 3T3 Cells Adaptor Proteins, Signal Transducing / genetics Adaptor Proteins, Signal Transducing / metabolism* Animals Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Cell Differentiation* Mesenchymal Stem Cells / metabolism* Mesenchymal Stem Cells / pathology Mice MicroRNAs / genetics MicroRNAs / metabolism Osteoblasts / metabolism* Osteoblasts / pathology Osteogenesis* Osteoporosis / genetics Osteoporosis / metabolism* Osteoporosis / pathology RNA, Circular / genetics RNA, Circular / metabolism Up-Regulation Wnt Signaling Pathway* YAP-Signaling Proteins |
| IF | 4.545 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |