論文 - 詳細
| RRC ID | 6330 |
|---|---|
| 著者 | Buck SH, Chiu D, Saito RM. |
| タイトル | The cyclin-dependent kinase inhibitors, cki-1 and cki-2, act in overlapping but distinct pathways to control cell cycle quiescence during C. elegans development. |
| ジャーナル | Cell Cycle |
| Abstract |
Cyclin-dependent kinase inhibitors (CKIs) are major contributors to the decision to enter or exit the cell cycle. The Caenorhabditis elegans genome encodes two CKIs belonging to the Cip/Kip family, cki-1 and cki-2. cki-1 has been shown to act as a canonical negative regulator of cell cycle entry, while the role of cki-2 remains unclear. We identified cki-2 in a genome-wide RNAi screen to reveal genes essential for developmental cell cycle quiescence. Examination of cki-2 knockout animals revealed extra rounds of cell divisions, verifying a role in establishing or maintaining the temporary cell cycle arrest. Despite the overlapping defects, the pathways mediated by cki-1 and cki-2 are discrete since the extra cell phenotype conferred by a putative cki-2(null) mutation is enhanced upon additional loss of cki-1 activity. Moreover, the extra cell division defect of cki-2 is not increased with the additional loss of lin-35 Rb, as is seen with cki-1. Thus, both cki-1 and cki-2 mediate cell cycle quiescence, but our genetic and phenotypic analyses demonstrate that they act within distinct pathways to exert control over the cell cycle machinery. |
| 巻・号 | 8(16) |
| ページ | 2613-20 |
| 公開日 | 2009-8-15 |
| DOI | 10.4161/cc.8.16.9354 |
| PII | 9354 |
| PMID | 19597327 |
| PMC | PMC3141283 |
| MeSH | Animals Animals, Genetically Modified Caenorhabditis elegans / embryology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / physiology* Cell Cycle / genetics Cell Cycle / physiology* Cyclin-Dependent Kinase Inhibitor Proteins / genetics Cyclin-Dependent Kinase Inhibitor Proteins / physiology* Gene Expression Regulation, Developmental / genetics Gene Expression Regulation, Developmental / physiology Mutation Phenotype Polymerase Chain Reaction RNA, Bacterial |
| IF | 3.699 |
| 引用数 | 20 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 線虫 | tm3496 |