論文 - 詳細
| RRC ID | 63388 |
|---|---|
| 著者 | Fukuda T, Iino Y, Onuma M, Gen B, Inoue-Murayama M, Kiyono T. |
| タイトル | Expression of human cell cycle regulators in the primary cell line of the African savannah elephant (loxodonta africana) increases proliferation until senescence, but does not induce immortalization. |
| ジャーナル | In Vitro Cell Dev Biol Anim |
| Abstract |
The African savannah elephant (Loxodonta africana) is one of the critically endangered animals. Conservation of genetic and cellular resources is important for the promotion of wild life-related research. Although primary cultured cells are a useful model for the physiology and genomics of the wild-type animals, their distribution is restricted due to the limited number of cell divisions allowed in them. Here, we tried to immortalize a primary cell line of L. africana with by overexpressing human mutant form of cyclin-dependent kinase 4 (CDK4R24C), cyclin D, and telomerase (TERT). It has been shown before that the combination of human CDK4R24C, cyclin D, and TERT induces the efficient cellular immortalization of cells derived from humans, bovine, swine, and monkeys. Interestingly, although the combination of these three genes extended the cellular proliferation of the L. africana-derived cells, they did not induce cellular immortalization. This study suggest that control of cellular senescence in L. africana-derived cells would be different molecular mechanisms compared to those governing human, bovine, swine, and monkey cells. |
| 巻・号 | 52(1) |
| ページ | 20-6 |
| 公開日 | 2016-1-1 |
| DOI | 10.1007/s11626-015-9943-6 |
| PII | 10.1007/s11626-015-9943-6 |
| PMID | 26487427 |
| MeSH | Animals Cattle Cell Cycle Proteins / biosynthesis* Cell Proliferation / genetics* Cellular Senescence / genetics* Conservation of Natural Resources Cyclin D / biosynthesis Cyclin D / genetics Cyclin-Dependent Kinase 4 / genetics Elephants / genetics* Endangered Species Gene Expression Regulation Humans Primary Cell Culture* Telomerase / biosynthesis Telomerase / genetics |
| IF | 1.665 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | LACF-NaNaII(RCB2320) |