論文 - 詳細
| RRC ID | 6341 |
|---|---|
| 著者 | Chen C, Tuck S, Byström AS. |
| タイトル | Defects in tRNA modification associated with neurological and developmental dysfunctions in Caenorhabditis elegans elongator mutants. |
| ジャーナル | PLoS Genet |
| Abstract |
Elongator is a six subunit protein complex, conserved from yeast to humans. Mutations in the human Elongator homologue, hELP1, are associated with the neurological disease familial dysautonomia. However, how Elongator functions in metazoans, and how the human mutations affect neural functions is incompletely understood. Here we show that in Caenorhabditis elegans, ELPC-1 and ELPC-3, components of the Elongator complex, are required for the formation of the 5-carbamoylmethyl and 5-methylcarboxymethyl side chains of wobble uridines in tRNA. The lack of these modifications leads to defects in translation in C. elegans. ELPC-1::GFP and ELPC-3::GFP reporters are strongly expressed in a subset of chemosensory neurons required for salt chemotaxis learning. elpc-1 or elpc-3 gene inactivation causes a defect in this process, associated with a posttranscriptional reduction of neuropeptide and a decreased accumulation of acetylcholine in the synaptic cleft. elpc-1 and elpc-3 mutations are synthetic lethal together with those in tuc-1, which is required for thiolation of tRNAs having the 5'methylcarboxymethyl side chain. elpc-1; tuc-1 and elpc-3; tuc-1 double mutants display developmental defects. Our results suggest that, by its effect on tRNA modification, Elongator promotes both neural function and development. |
| 巻・号 | 5(7) |
| ページ | e1000561 |
| 公開日 | 2009-7-1 |
| DOI | 10.1371/journal.pgen.1000561 |
| PMID | 19593383 |
| PMC | PMC2702823 |
| MeSH | Acetylation Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / growth & development Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Chemotaxis Cholinesterase Inhibitors Fertility Gene Expression Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Life Cycle Stages Microscopy, Fluorescence Mutation Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism Nucleic Acid Conformation Protein Biosynthesis RNA, Transfer / metabolism* Temperature Tubulin / metabolism Uridine / analogs & derivatives Uridine / metabolism |
| IF | 5.175 |
| 引用数 | 92 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2149 tm3120 tm1297 |