Reference - Detail
| RRC ID | 63460 |
|---|---|
| Author | Mori K, Sun H, Miura K, Simizu S. |
| Title | Involvement of DPY19L3 in Myogenic Differentiation of C2C12 Myoblasts. |
| Journal | Molecules |
| Abstract |
DPY19L3 has been identified as a C-mannosyltransferase for thrombospondin type-1 repeat domain-containing proteins. In this study, we focused on the role of DPY19L3 in the myogenic differentiation of C2C12 mouse myoblast cells. We carried out DPY19L3 gene depletion using the CRISPR/Cas9 system. The result showed that these DPY19L3-knockout cells could not be induced for differentiation. Moreover, the phosphorylation levels of MEK/ERK and p70S6K were suppressed in the DPY19L3-knockout cells compared with that of parent cells, suggesting that the protein(s) that is(are) DPY19L3-mediated C-mannosylated and regulate(s) MEK/ERK or p70S6K signaling is(are) required for the differentiation. |
| Volume | 26(18) |
| Published | 2021-9-19 |
| DOI | 10.3390/molecules26185685 |
| PII | molecules26185685 |
| PMID | 34577156 |
| PMC | PMC8467457 |
| MeSH | Animals Cell Differentiation / genetics* Cell Differentiation / physiology* Cell Line Extracellular Signal-Regulated MAP Kinases / metabolism Gene Knockdown Techniques Glycosylation Mannosyltransferases / genetics Mannosyltransferases / physiology* Mice Mitogen-Activated Protein Kinase Kinases / metabolism Myoblasts / cytology Myoblasts / physiology* Phosphorylation / genetics Ribosomal Protein S6 Kinases, 70-kDa / metabolism Signal Transduction / genetics Up-Regulation / genetics |
| IF | 3.267 |
| Resource | |
| Human and Animal Cells | C2C12(RCB0987) |