論文 - 詳細
| RRC ID | 63466 |
|---|---|
| 著者 | Bellec K, Pinot M, Gicquel I, Le Borgne R. |
| タイトル | The Clathrin adaptor AP-1 and Stratum act in parallel pathways to control Notch activation in Drosophila sensory organ precursors cells. |
| ジャーナル | Development |
| Abstract |
Drosophila sensory organ precursors divide asymmetrically to generate pIIa/pIIb cells, the identity of which relies on activation of Notch at cytokinesis. Although Notch is present apically and basally relative to the midbody at the pIIa-pIIb interface, the basal pool of Notch is reported to be the main contributor for Notch activation in the pIIa cell. Intra-lineage signalling requires appropriate apico-basal targeting of Notch, its ligand Delta and its trafficking partner Sanpodo. We have previously reported that AP-1 and Stratum regulate the trafficking of Notch and Sanpodo from the trans-Golgi network to the basolateral membrane. Loss of AP-1 or Stratum caused mild Notch gain-of-function phenotypes. Here, we report that their concomitant loss results in a penetrant Notch gain-of-function phenotype, indicating that they control parallel pathways. Although unequal partitioning of cell fate determinants and cell polarity were unaffected, we observed increased amounts of signalling-competent Notch as well as Delta and Sanpodo at the apical pIIa-pIIb interface, at the expense of the basal pool of Notch. We propose that AP-1 and Stratum operate in parallel pathways to localize Notch and control where receptor activation takes place. |
| 巻・号 | 148(1) |
| 公開日 | 2021-1-10 |
| DOI | 10.1242/dev.191437 |
| PII | dev.191437 |
| PMID | 33298463 |
| PMC | PMC7823167 |
| MeSH | Adaptor Protein Complex 1 / metabolism* Animals Cell Lineage Cell Nucleus / metabolism Cell Polarity Drosophila Proteins / metabolism* Drosophila melanogaster / cytology* Drosophila melanogaster / metabolism* Gain of Function Mutation Guanine Nucleotide Exchange Factors / metabolism* Penetrance Phenotype Receptors, Notch / metabolism* Sense Organs / metabolism* Stem Cells / metabolism* |
| IF | 5.611 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | |