RRC ID 63466
Author Bellec K, Pinot M, Gicquel I, Le Borgne R.
Title The Clathrin adaptor AP-1 and Stratum act in parallel pathways to control Notch activation in Drosophila sensory organ precursors cells.
Journal Development
Abstract Drosophila sensory organ precursors divide asymmetrically to generate pIIa/pIIb cells, the identity of which relies on activation of Notch at cytokinesis. Although Notch is present apically and basally relative to the midbody at the pIIa-pIIb interface, the basal pool of Notch is reported to be the main contributor for Notch activation in the pIIa cell. Intra-lineage signalling requires appropriate apico-basal targeting of Notch, its ligand Delta and its trafficking partner Sanpodo. We have previously reported that AP-1 and Stratum regulate the trafficking of Notch and Sanpodo from the trans-Golgi network to the basolateral membrane. Loss of AP-1 or Stratum caused mild Notch gain-of-function phenotypes. Here, we report that their concomitant loss results in a penetrant Notch gain-of-function phenotype, indicating that they control parallel pathways. Although unequal partitioning of cell fate determinants and cell polarity were unaffected, we observed increased amounts of signalling-competent Notch as well as Delta and Sanpodo at the apical pIIa-pIIb interface, at the expense of the basal pool of Notch. We propose that AP-1 and Stratum operate in parallel pathways to localize Notch and control where receptor activation takes place.
Volume 148(1)
Published 2021-1-10
DOI 10.1242/dev.191437
PII dev.191437
PMID 33298463
PMC PMC7823167
MeSH Adaptor Protein Complex 1 / metabolism* Animals Cell Lineage Cell Nucleus / metabolism Cell Polarity Drosophila Proteins / metabolism* Drosophila melanogaster / cytology* Drosophila melanogaster / metabolism* Gain of Function Mutation Guanine Nucleotide Exchange Factors / metabolism* Penetrance Phenotype Receptors, Notch / metabolism* Sense Organs / metabolism* Stem Cells / metabolism*
IF 5.611