| RRC ID |
63695
|
| 著者 |
Nambara S, Masuda T, Kobayashi Y, Sato K, Tobo T, Koike K, Noda M, Ogawa Y, Kuroda Y, Ito S, Eguchi H, Sugimachi K, Mimori K.
|
| タイトル |
GTF2IRD1 on chromosome 7 is a novel oncogene regulating the tumor-suppressor gene TGFβR2 in colorectal cancer.
|
| ジャーナル |
Cancer Sci
|
| Abstract |
Chromosome 7q (Ch.7q) is clonally amplified in colorectal cancer (CRC). We aimed to identify oncogenes on Ch.7q that are overexpressed through DNA copy number amplification and determine the biological and clinical significance of these oncogenes in CRC. We identified general transcription factor 2I repeat domain-containing protein 1 (GTF2IRD1) as a potential oncogene using a CRC dataset from The Cancer Genome Atlas with a bioinformatics approach. We measured the expression of GTF2IRD1 in 98 patients with CRC using immunohistochemistry and RT-quantitative PCR (RT-qPCR). The biological effects of GTF2IRD1 expression were explored by gene set enrichment analysis (GSEA). Next, we undertook in vitro cell proliferation and cell cycle assays using siGTF2IRD1-transfected CRC cells. We further investigated the oncogenic mechanisms through which GTF2IRD1 promoted CRC progression. Finally, we assessed the clinical significance of GTF2IRD1 expression by RT-qPCR. GTF2IRD1 was overexpressed in tumor cells and liver metastatic lesions. The GSEA revealed a positive correlation between GTF2IRD1 expression and cell cycle progression-related genes. GTF2IRD1 knockdown inhibited cell proliferation and induced cell cycle arrest in Smad4-mutated CRC. GTF2IRD1 downregulated the expression of the gene encoding transforming growth factor β receptor 2 (TGFβR2), a tumor-suppressor gene in Smad4-mutated CRC. On multivariate analysis, high GTF2IRD1 expression was an independent poor prognostic factor. Clinicopathological analysis showed that GTF2IRD1 expression was positively correlated with liver metastasis. In conclusion, GTF2IRD1 promoted CRC progression by downregulating TGFβR2 and could be a prognostic biomarker on Ch.7q in CRC. GTF2IRD1 could also be a novel oncogene in CRC.
|
| 巻・号 |
111(2)
|
| ページ |
343-355
|
| 公開日 |
2020-2-1
|
| DOI |
10.1111/cas.14248
|
| PMID |
31758608
|
| PMC |
PMC7004548
|
| MeSH |
Biomarkers, Tumor / genetics
Biomarkers, Tumor / metabolism
Cell Cycle
Cell Line, Tumor
Cell Proliferation
Chromosomes, Human, Pair 7 / genetics
Colorectal Neoplasms / genetics*
Colorectal Neoplasms / metabolism
Female
Gene Amplification
Gene Expression Regulation, Neoplastic
HCT116 Cells
Humans
Liver Neoplasms / genetics*
Liver Neoplasms / metabolism
Liver Neoplasms / secondary*
Male
Muscle Proteins / genetics*
Muscle Proteins / metabolism*
Nuclear Proteins / genetics*
Nuclear Proteins / metabolism*
Prognosis
Receptor, Transforming Growth Factor-beta Type II / metabolism*
Trans-Activators / genetics*
Trans-Activators / metabolism*
Up-Regulation
|
| IF |
4.966
|
| リソース情報 |
| ヒト・動物細胞 |
COLO205(RCB2127)
COLO-320(RCB1193)
HCT116(RCB2979)
LoVo(RCB1639) |
