RRC ID 6370
Author Han T, Manoharan AP, Harkins TT, Bouffard P, Fitzpatrick C, Chu DS, Thierry-Mieg D, Thierry-Mieg J, Kim JK.
Title 26G endo-siRNAs regulate spermatogenic and zygotic gene expression in Caenorhabditis elegans.
Journal Proc. Natl. Acad. Sci. U.S.A.
Abstract Endogenous small interfering RNAs (endo-siRNAs) regulate diverse gene expression programs in eukaryotes by either binding and cleaving mRNA targets or mediating heterochromatin formation; however, the mechanisms of endo-siRNA biogenesis, sorting, and target regulation remain poorly understood. Here we report the identification and function of a specific class of germline-generated endo-siRNAs in Caenorhabditis elegans that are 26 nt in length and contain a guanine at the first nucleotide position (i.e., 26G RNAs). 26G RNAs regulate gene expression during spermatogenesis and zygotic development, and their biogenesis requires the ERI-1 exonuclease and the RRF-3 RNA-dependent RNA polymerase (RdRP). Remarkably, we identified two nonoverlapping subclasses of 26G RNAs that sort into specific RNA-induced silencing complexes (RISCs) and differentially regulate distinct mRNA targets. Class I 26G RNAs target genes are expressed during spermatogenesis, whereas class II 26G RNAs are maternally inherited and silence gene expression during zygotic development. These findings implicate a class of endo-siRNAs in the global regulation of transcriptional programs required for fertility and development.
Volume 106(44)
Pages 18674-9
Published 2009-11-3
DOI 10.1073/pnas.0906378106
PII 0906378106
PMID 19846761
PMC PMC2765456
MeSH Animals Caenorhabditis elegans / embryology* Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / metabolism Exoribonucleases / metabolism Gene Expression Regulation, Developmental* Gene Silencing Germ Cells / metabolism Guanine / metabolism* Male RNA, Helminth / classification RNA, Helminth / metabolism RNA, Small Interfering / biosynthesis RNA, Small Interfering / classification RNA, Small Interfering / metabolism* Sequence Analysis, DNA Spermatogenesis / genetics* Zygote / metabolism*
IF 9.504
Times Cited 72
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
C.elegans tm1860 tm1155 tm1184