論文 - 詳細
| RRC ID | 6370 |
|---|---|
| 著者 | Han T, Manoharan AP, Harkins TT, Bouffard P, Fitzpatrick C, Chu DS, Thierry-Mieg D, Thierry-Mieg J, Kim JK. |
| タイトル | 26G endo-siRNAs regulate spermatogenic and zygotic gene expression in Caenorhabditis elegans. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Endogenous small interfering RNAs (endo-siRNAs) regulate diverse gene expression programs in eukaryotes by either binding and cleaving mRNA targets or mediating heterochromatin formation; however, the mechanisms of endo-siRNA biogenesis, sorting, and target regulation remain poorly understood. Here we report the identification and function of a specific class of germline-generated endo-siRNAs in Caenorhabditis elegans that are 26 nt in length and contain a guanine at the first nucleotide position (i.e., 26G RNAs). 26G RNAs regulate gene expression during spermatogenesis and zygotic development, and their biogenesis requires the ERI-1 exonuclease and the RRF-3 RNA-dependent RNA polymerase (RdRP). Remarkably, we identified two nonoverlapping subclasses of 26G RNAs that sort into specific RNA-induced silencing complexes (RISCs) and differentially regulate distinct mRNA targets. Class I 26G RNAs target genes are expressed during spermatogenesis, whereas class II 26G RNAs are maternally inherited and silence gene expression during zygotic development. These findings implicate a class of endo-siRNAs in the global regulation of transcriptional programs required for fertility and development. |
| 巻・号 | 106(44) |
| ページ | 18674-9 |
| 公開日 | 2009-11-3 |
| DOI | 10.1073/pnas.0906378106 |
| PII | 0906378106 |
| PMID | 19846761 |
| PMC | PMC2765456 |
| MeSH | Animals Caenorhabditis elegans / embryology* Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / metabolism Exoribonucleases / metabolism Gene Expression Regulation, Developmental* Gene Silencing Germ Cells / metabolism Guanine / metabolism* Male RNA, Helminth / classification RNA, Helminth / metabolism RNA, Small Interfering / biosynthesis RNA, Small Interfering / classification RNA, Small Interfering / metabolism* Sequence Analysis, DNA Spermatogenesis / genetics* Zygote / metabolism* |
| IF | 9.412 |
| 引用数 | 92 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1860 tm1155 tm1184 |