| RRC ID |
6387
|
| 著者 |
King RS, Maiden SL, Hawkins NC, Kidd AR 3rd, Kimble J, Hardin J, Walston TD.
|
| タイトル |
The N- or C-terminal domains of DSH-2 can activate the C. elegans Wnt/beta-catenin asymmetry pathway.
|
| ジャーナル |
Dev Biol
|
| Abstract |
Dishevelleds are modular proteins that lie at the crossroads of divergent Wnt signaling pathways. The DIX domain of dishevelleds modulates a beta-catenin destruction complex, and thereby mediates cell fate decisions through differential activation of Tcf transcription factors. The DEP domain of dishevelleds mediates planar polarity of cells within a sheet through regulation of actin modulators. In Caenorhabditis elegans asymmetric cell fate decisions are regulated by asymmetric localization of signaling components in a pathway termed the Wnt/beta-catenin asymmetry pathway. Which domain(s) of Disheveled regulate this pathway is unknown. We show that C. elegans embryos from dsh-2(or302) mutant mothers fail to successfully undergo morphogenesis, but transgenes containing either the DIX or the DEP domain of DSH-2 are sufficient to rescue the mutant phenotype. Embryos lacking zygotic function of SYS-1/beta-catenin, WRM-1/beta-catenin, or POP-1/Tcf show defects similar to dsh-2 mutants, including a loss of asymmetry in some cell fate decisions. Removal of two dishevelleds (dsh-2 and mig-5) leads to a global loss of POP-1 asymmetry, which can be rescued by addition of transgenes containing either the DIX or DEP domain of DSH-2. These results indicate that either the DIX or DEP domain of DSH-2 is capable of activating the Wnt/beta-catenin asymmetry pathway and regulating anterior-posterior fate decisions required for proper morphogenesis.
|
| 巻・号 |
328(2)
|
| ページ |
234-44
|
| 公開日 |
2009-4-15
|
| DOI |
10.1016/j.ydbio.2009.01.017
|
| PII |
S0012-1606(09)00054-2
|
| PMID |
19298786
|
| PMC |
PMC4409331
|
| MeSH |
Animals
Animals, Genetically Modified
Body Patterning / physiology
Caenorhabditis elegans / cytology
Caenorhabditis elegans / embryology
Caenorhabditis elegans / physiology*
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / physiology*
Cell Cycle Proteins / genetics
Cell Cycle Proteins / physiology*
Cell Polarity / physiology
Cytoskeletal Proteins / physiology*
DNA-Binding Proteins / physiology
Dishevelled Proteins
Embryo, Nonmammalian / physiology
High Mobility Group Proteins / physiology
Mutation
Protein Structure, Tertiary
Signal Transduction / physiology
Transcription Factors / physiology*
Wnt Proteins / physiology*
beta Catenin / physiology*
|
| IF |
2.896
|
| 引用数 |
17
|
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
|
| リソース情報 |
| 線虫 |
tm2133 |
