論文 - 詳細
| RRC ID | 6387 |
|---|---|
| 著者 | King RS, Maiden SL, Hawkins NC, Kidd AR 3rd, Kimble J, Hardin J, Walston TD. |
| タイトル | The N- or C-terminal domains of DSH-2 can activate the C. elegans Wnt/beta-catenin asymmetry pathway. |
| ジャーナル | Dev Biol |
| Abstract |
Dishevelleds are modular proteins that lie at the crossroads of divergent Wnt signaling pathways. The DIX domain of dishevelleds modulates a beta-catenin destruction complex, and thereby mediates cell fate decisions through differential activation of Tcf transcription factors. The DEP domain of dishevelleds mediates planar polarity of cells within a sheet through regulation of actin modulators. In Caenorhabditis elegans asymmetric cell fate decisions are regulated by asymmetric localization of signaling components in a pathway termed the Wnt/beta-catenin asymmetry pathway. Which domain(s) of Disheveled regulate this pathway is unknown. We show that C. elegans embryos from dsh-2(or302) mutant mothers fail to successfully undergo morphogenesis, but transgenes containing either the DIX or the DEP domain of DSH-2 are sufficient to rescue the mutant phenotype. Embryos lacking zygotic function of SYS-1/beta-catenin, WRM-1/beta-catenin, or POP-1/Tcf show defects similar to dsh-2 mutants, including a loss of asymmetry in some cell fate decisions. Removal of two dishevelleds (dsh-2 and mig-5) leads to a global loss of POP-1 asymmetry, which can be rescued by addition of transgenes containing either the DIX or DEP domain of DSH-2. These results indicate that either the DIX or DEP domain of DSH-2 is capable of activating the Wnt/beta-catenin asymmetry pathway and regulating anterior-posterior fate decisions required for proper morphogenesis. |
| 巻・号 | 328(2) |
| ページ | 234-44 |
| 公開日 | 2009-4-15 |
| DOI | 10.1016/j.ydbio.2009.01.017 |
| PII | S0012-1606(09)00054-2 |
| PMID | 19298786 |
| PMC | PMC4409331 |
| MeSH | Animals Animals, Genetically Modified Body Patterning / physiology Caenorhabditis elegans / cytology Caenorhabditis elegans / embryology Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / physiology* Cell Cycle Proteins / genetics Cell Cycle Proteins / physiology* Cell Polarity / physiology Cytoskeletal Proteins / physiology* DNA-Binding Proteins / physiology Dishevelled Proteins Embryo, Nonmammalian / physiology High Mobility Group Proteins / physiology Mutation Protein Structure, Tertiary Signal Transduction / physiology Transcription Factors / physiology* Wnt Proteins / physiology* beta Catenin / physiology* |
| IF | 2.896 |
| 引用数 | 17 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 線虫 | tm2133 |