論文 - 詳細
| RRC ID | 6391 |
|---|---|
| 著者 | Lesch BJ, Gehrke AR, Bulyk ML, Bargmann CI. |
| タイトル | Transcriptional regulation and stabilization of left-right neuronal identity in C. elegans. |
| ジャーナル | Genes Dev |
| Abstract |
At discrete points in development, transient signals are transformed into long-lasting cell fates. For example, the asymmetric identities of two Caenorhabditis elegans olfactory neurons called AWC(ON) and AWC(OFF) are specified by an embryonic signaling pathway, but maintained throughout the life of an animal. Here we show that the DNA-binding protein NSY-7 acts to convert a transient, partially differentiated state into a stable AWC(ON) identity. Expression of an AWC(ON) marker is initiated in nsy-7 loss-of-function mutants, but subsequently lost, so that most adult animals have two AWC(OFF) neurons and no AWC(ON) neurons. nsy-7 encodes a protein with distant similarity to a homeodomain. It is expressed in AWC(ON), and is an early transcriptional target of the embryonic signaling pathway that specifies AWC(ON) and AWC(OFF); its expression anticipates future AWC asymmetry. The NSY-7 protein binds a specific optimal DNA sequence that was identified through a complete biochemical survey of 8-mer DNA sequences. This sequence is present in the promoter of an AWC(OFF) marker and essential for its asymmetric expression. An 11-base-pair (bp) sequence required for AWC(OFF) expression has two activities: One region activates expression in both AWCs, and the overlapping NSY-7-binding site inhibits expression in AWC(ON). Our results suggest that NSY-7 responds to transient embryonic signaling by repressing AWC(OFF) genes in AWC(ON), thus acting as a transcriptional selector for a randomly specified neuronal identity. |
| 巻・号 | 23(3) |
| ページ | 345-58 |
| 公開日 | 2009-2-1 |
| DOI | 10.1101/gad.1763509 |
| PII | 23/3/345 |
| PMID | 19204119 |
| PMC | PMC2648548 |
| MeSH | Amino Acid Sequence Animals Base Sequence Body Patterning / genetics* Body Patterning / physiology Caenorhabditis elegans / genetics* Caenorhabditis elegans / growth & development* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Consensus Sequence Cyclic GMP / metabolism DNA, Helminth / genetics DNA, Helminth / metabolism Gene Expression Regulation, Developmental Genes, Helminth Homeodomain Proteins / genetics Homeodomain Proteins / metabolism Larva / growth & development Larva / metabolism Molecular Sequence Data Mutation Nuclear Proteins / genetics Nuclear Proteins / metabolism Olfactory Receptor Neurons / growth & development Olfactory Receptor Neurons / metabolism Sequence Homology, Amino Acid Signal Transduction |
| IF | 9.527 |
| 引用数 | 38 |
| WOS 分野 | GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3080 |