RRC ID 6400
著者 Rolland SG, Lu Y, David CN, Conradt B.
タイトル The BCL-2-like protein CED-9 of C. elegans promotes FZO-1/Mfn1,2- and EAT-3/Opa1-dependent mitochondrial fusion.
ジャーナル J Cell Biol
Abstract The mammalian dynamin-related guanosine triphosphatases Mfn1,2 and Opa1 are required for mitochondrial fusion. However, how their activities are controlled and coordinated is largely unknown. We present data that implicate the BCL-2-like protein CED-9 in the control of mitochondrial fusion in Caenorhabditis elegans. We demonstrate that CED-9 can promote complete mitochondrial fusion of both the outer and inner mitochondrial membrane. We also show that this fusion is dependent on the C. elegans Mfn1,2 homologue FZO-1 and the C. elegans Opa1 homologue EAT-3. Furthermore, we show that CED-9 physically interacts with FZO-1 in vivo and that the ability of CED-9 to interact with FZO-1 is important for its ability to cause mitochondrial fusion. CED-9-induced mitochondrial fusion is not required for the maintenance of mitochondrial morphology during embryogenesis or in muscle cells, at least under normal conditions and in the absence of stress. Therefore, we propose that the BCL-2-like CED-9 acts through FZO-1/Mfn1,2 and EAT-3/Opa1 to promote mitochondrial fusion in response to specific cellular signals.
巻・号 186(4)
ページ 525-40
公開日 2009-8-24
DOI 10.1083/jcb.200905070
PII jcb.200905070
PMID 19704021
PMC PMC2733758
MeSH Animals Animals, Genetically Modified Apoptosis Regulatory Proteins / genetics Apoptosis Regulatory Proteins / metabolism Caenorhabditis elegans / cytology Caenorhabditis elegans / embryology Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Calcium-Calmodulin-Dependent Protein Kinases / genetics Calcium-Calmodulin-Dependent Protein Kinases / metabolism Death-Associated Protein Kinases GTP Phosphohydrolases / genetics GTP Phosphohydrolases / metabolism* Membrane Fusion / physiology* Mitochondria / metabolism* Mitochondria / ultrastructure Mitochondrial Membranes / metabolism Mutation Proto-Oncogene Proteins c-bcl-2 / genetics Proto-Oncogene Proteins c-bcl-2 / metabolism* RNA Interference Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Two-Hybrid System Techniques
IF 8.811
引用数 61
WOS 分野 CELL BIOLOGY
リソース情報
線虫 tm1133 tm1108