RRC ID 64024
Author Koga K, Aoki M, Sameshima T, Hamasaki M, Egawa N, Seiki M, Toole BP, Suzumiya J, Nabeshima K.
Title Synthetic emmprin peptides inhibit tumor cell-fibroblast interaction-stimulated upregulation of MMP-2 and tumor cell invasion.
Journal Int J Oncol
Abstract Stromal cells are the main source of matrix metalloproteinases (MMPs) in human carcinoma tissues. Emmprin is a glycosylated transmembrane protein containing two immunoglobulin (Ig) domains that is expressed in carcinoma cells and stimulates MMP production by adjacent stromal cells. The first Ig domain (ECI) of emmprin contains the biologically active site. We investigated whether synthetic peptides carrying a partial ECI sequence could inhibit emmprin activity. Only the second peptide (emp#2), which contains a putative N-glycosylation site sequence, inhibited emmprin-stimulated production of MMP-2 in co-cultures of fibroblasts and several different human tumor cells types, including carcinoma, sarcoma, melanoma, leukemia and glioma cells. Moreover, emp#2 significantly inhibited the invasive activity of glioblastoma cells promoted by interaction with fibroblasts. Perturbation of emmprin activity by this peptide may have potential therapeutic uses in the prevention of MMP-2-dependent cancer invasion.
Volume 39(3)
Pages 657-64
Published 2011-9-1
DOI 10.3892/ijo.2011.1060
PMID 21637915
MeSH Amino Acid Sequence Basigin / chemistry Basigin / metabolism Basigin / pharmacology* Cell Communication / drug effects* Cell Line, Tumor Coculture Techniques Fibroblasts / enzymology Fibroblasts / pathology* Glioblastoma / enzymology Glioblastoma / genetics Glioblastoma / pathology HEK293 Cells Humans Matrix Metalloproteinase 2 / biosynthesis* Matrix Metalloproteinase 2 / genetics Matrix Metalloproteinase 2 / metabolism Molecular Sequence Data Neoplasm Invasiveness Neoplasms / enzymology Neoplasms / genetics Neoplasms / pathology* Peptide Fragments / chemistry Peptide Fragments / pharmacology* Up-Regulation / drug effects
IF 3.899
Resource
Human and Animal Cells U251(RCB0461)