論文 - 詳細
| RRC ID | 64088 |
|---|---|
| 著者 | Kramarz K, Schirmeisen K, Boucherit V, Ait Saada A, Lovo C, Palancade B, Freudenreich C, Lambert SAE. |
| タイトル | The nuclear pore primes recombination-dependent DNA synthesis at arrested forks by promoting SUMO removal. |
| ジャーナル | Nat Commun |
| Abstract |
Nuclear Pore complexes (NPCs) act as docking sites to anchor particular DNA lesions facilitating DNA repair by elusive mechanisms. Using replication fork barriers in fission yeast, we report that relocation of arrested forks to NPCs occurred after Rad51 loading and its enzymatic activity. The E3 SUMO ligase Pli1 acts at arrested forks to safeguard integrity of nascent strands and generates poly-SUMOylation which promote relocation to NPCs but impede the resumption of DNA synthesis by homologous recombination (HR). Anchorage to NPCs allows SUMO removal by the SENP SUMO protease Ulp1 and the proteasome, promoting timely resumption of DNA synthesis. Preventing Pli1-mediated SUMO chains was sufficient to bypass the need for anchorage to NPCs and the inhibitory effect of poly-SUMOylation on HR-mediated DNA synthesis. Our work establishes a novel spatial control of Recombination-Dependent Replication (RDR) at a unique sequence that is distinct from mechanisms engaged at collapsed-forks and breaks within repeated sequences. |
| 巻・号 | 11(1) |
| ページ | 5643 |
| 公開日 | 2020-11-6 |
| DOI | 10.1038/s41467-020-19516-z |
| PII | 10.1038/s41467-020-19516-z |
| PMID | 33159083 |
| PMC | PMC7648084 |
| MeSH | DNA Replication Ligases / genetics Ligases / metabolism Nuclear Pore / genetics Nuclear Pore / metabolism* Proteasome Endopeptidase Complex / genetics* Proteasome Endopeptidase Complex / metabolism Recombination, Genetic Schizosaccharomyces / genetics* Schizosaccharomyces / growth & development Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Small Ubiquitin-Related Modifier Proteins / genetics Small Ubiquitin-Related Modifier Proteins / metabolism* Transcription, Genetic |
| IF | 12.121 |
| オルトメトリクス指標 |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 11 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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