RRC ID 64105
Author Tsujimoto H, Morimoto Y, Takahata R, Nomura S, Yoshida K, Hiraki S, Horiguchi H, Miyazaki H, Ono S, Saito D, Hara I, Ozeki E, Yamamoto J, Hase K.
Title Theranostic Photosensitive Nanoparticles for Lymph Node Metastasis of Gastric Cancer.
Journal Ann Surg Oncol
Abstract BACKGROUND:Preoperative and intraoperative diagnoses of lymph node (LN) metastasis in patients with gastric cancer is essential to determine the extent of LN dissection in order to establish individualized treatment strategies. We investigated the theranostic value of a newly developed drug delivery system employing nanoparticles loaded with the indocyanine green (ICG) derivative ICG-loaded lactosome (ICGm) using a murine draining LN metastasis model of gastric cancer.
METHODS:In the experimental draining LN metastasis model of human gastric cancer, the right hind footpads of nude mice were injected with cancer cells. Three weeks later, either ICGm or ICG solution was injected through the tail vein. Forty-eight hours after the administration of a photosensitizer, in vivo and ex vivo imaging and photodynamic therapy (PDT) were performed, and size of the LNs was measured.
RESULTS:In vivo imaging revealed metastatic LNs in the ICGm-treated mice but not in the ICG-treated mice. PDT using ICGm induced apoptosis and significantly inhibited the growth of metastatic LNs.
CONCLUSIONS:ICGm presents a novel theranostic nanodevice for LN metastasis of gastric cancer.
Volume 22 Suppl 3
Pages S923-8
Published 2015-12-1
DOI 10.1245/s10434-015-4594-0
PII 10.1245/s10434-015-4594-0
PMID 25986868
MeSH Adenocarcinoma / metabolism Adenocarcinoma / prevention & control Adenocarcinoma / secondary* Animals Apoptosis* Cell Proliferation Coloring Agents / metabolism Drug Delivery Systems Fluorescence Humans Indocyanine Green / metabolism Lactic Acid / administration & dosage Lymph Nodes / pathology* Lymphatic Metastasis Male Mice Mice, Inbred BALB C Mice, Nude Nanoparticles / administration & dosage* Photochemotherapy* Photosensitizing Agents / administration & dosage* Stomach Neoplasms / metabolism Stomach Neoplasms / pathology* Stomach Neoplasms / prevention & control Theranostic Nanomedicine* Tumor Cells, Cultured Xenograft Model Antitumor Assays
IF 4.061
Human and Animal Cells MKN45(RCB1001)