論文 - 詳細
| RRC ID | 6430 |
|---|---|
| 著者 | Pellegrino MW, Gasser RB, Sprenger F, Stetak A, Hajnal A. |
| タイトル | The conserved zinc finger protein VAB-23 is an essential regulator of epidermal morphogenesis in Caenorhabditis elegans. |
| ジャーナル | Dev Biol |
| Abstract |
Caenorhabditis elegans is an excellent model to observe cell movements and shape changes during the morphogenesis of the egg-shaped embryo into an elongated tube-like larva. Although much is known about the structural determinants involved in epidermal morphogenesis, relatively little is known about the transcriptional and post-transcriptional regulatory networks involved. Here, we describe the identification and functional characterization of the novel nuclear protein VAB-23, which belongs to a conserved protein family found in all metazoans. C. elegans VAB-23 is essential for ventral closure and elongation of the embryo. Time-lapse analysis indicates that VAB-23 is required for the formation of proper cell contacts between contralateral pairs of ventral epidermal cells. Tissue-specific rescue experiments reveal a function of VAB-23 in ventral neuroblasts that control the enclosure of the embryo by the overlaying epidermal cells. Finally, we provide evidence suggesting a role of VAB-23 in post-transcriptional gene regulation. We thus propose that VAB-23 regulates the expression of multiple secreted guidance cues in ventral neuroblasts that direct the migration of the overlaying epidermal cells. Members of the VAB-23 family may perform similar functions during morphogenesis in other metazoans. |
| 巻・号 | 336(1) |
| ページ | 84-93 |
| 公開日 | 2009-12-1 |
| DOI | 10.1016/j.ydbio.2009.09.036 |
| PII | S0012-1606(09)01221-4 |
| PMID | 19799893 |
| MeSH | Amino Acid Sequence Animals Caenorhabditis elegans / embryology Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology Carrier Proteins / genetics* Carrier Proteins / metabolism Carrier Proteins / physiology Conserved Sequence Embryo, Nonmammalian / embryology Embryo, Nonmammalian / metabolism Epidermis / embryology Epidermis / metabolism* Gene Expression Regulation, Developmental Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Immunohistochemistry Intracellular Signaling Peptides and Proteins Microscopy, Confocal Molecular Sequence Data Morphogenesis Nuclear Proteins / classification Nuclear Proteins / genetics Nuclear Proteins / metabolism Phylogeny Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Zinc Fingers* |
| IF | 2.896 |
| 引用数 | 3 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1945 |