| RRC ID |
6430
|
| 著者 |
Pellegrino MW, Gasser RB, Sprenger F, Stetak A, Hajnal A.
|
| タイトル |
The conserved zinc finger protein VAB-23 is an essential regulator of epidermal morphogenesis in Caenorhabditis elegans.
|
| ジャーナル |
Dev Biol
|
| Abstract |
Caenorhabditis elegans is an excellent model to observe cell movements and shape changes during the morphogenesis of the egg-shaped embryo into an elongated tube-like larva. Although much is known about the structural determinants involved in epidermal morphogenesis, relatively little is known about the transcriptional and post-transcriptional regulatory networks involved. Here, we describe the identification and functional characterization of the novel nuclear protein VAB-23, which belongs to a conserved protein family found in all metazoans. C. elegans VAB-23 is essential for ventral closure and elongation of the embryo. Time-lapse analysis indicates that VAB-23 is required for the formation of proper cell contacts between contralateral pairs of ventral epidermal cells. Tissue-specific rescue experiments reveal a function of VAB-23 in ventral neuroblasts that control the enclosure of the embryo by the overlaying epidermal cells. Finally, we provide evidence suggesting a role of VAB-23 in post-transcriptional gene regulation. We thus propose that VAB-23 regulates the expression of multiple secreted guidance cues in ventral neuroblasts that direct the migration of the overlaying epidermal cells. Members of the VAB-23 family may perform similar functions during morphogenesis in other metazoans.
|
| 巻・号 |
336(1)
|
| ページ |
84-93
|
| 公開日 |
2009-12-1
|
| DOI |
10.1016/j.ydbio.2009.09.036
|
| PII |
S0012-1606(09)01221-4
|
| PMID |
19799893
|
| MeSH |
Amino Acid Sequence
Animals
Caenorhabditis elegans / embryology
Caenorhabditis elegans / genetics*
Caenorhabditis elegans / metabolism
Caenorhabditis elegans Proteins / genetics*
Caenorhabditis elegans Proteins / metabolism
Caenorhabditis elegans Proteins / physiology
Carrier Proteins / genetics*
Carrier Proteins / metabolism
Carrier Proteins / physiology
Conserved Sequence
Embryo, Nonmammalian / embryology
Embryo, Nonmammalian / metabolism
Epidermis / embryology
Epidermis / metabolism*
Gene Expression Regulation, Developmental
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Immunohistochemistry
Intracellular Signaling Peptides and Proteins
Microscopy, Confocal
Molecular Sequence Data
Morphogenesis
Nuclear Proteins / classification
Nuclear Proteins / genetics
Nuclear Proteins / metabolism
Phylogeny
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
Zinc Fingers*
|
| IF |
2.896
|
| 引用数 |
3
|
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
|
| リソース情報 |
| 線虫 |
tm1945 |
