論文 - 詳細
| RRC ID | 64390 |
|---|---|
| 著者 | Ohhata T, Yamazawa K, Miura-Kamio A, Takahashi S, Sakai S, Tamura Y, Uchida C, Kitagawa K, Niida H, Hiratani I, Kobayashi H, Kimura H, Wutz A, Kitagawa M. |
| タイトル | Dynamics of transcription-mediated conversion from euchromatin to facultative heterochromatin at the Xist promoter by Tsix. |
| ジャーナル | Cell Rep |
| Abstract |
The fine-scale dynamics from euchromatin (EC) to facultative heterochromatin (fHC) has remained largely unclear. Here, we focus on Xist and its silencing initiator Tsix as a paradigm of transcription-mediated conversion from EC to fHC. In mouse epiblast stem cells, induction of Tsix recapitulates the conversion at the Xist promoter. Investigating the dynamics reveals that the conversion proceeds in a stepwise manner. Initially, a transient opened chromatin structure is observed. In the second step, gene silencing is initiated and dependent on Tsix, which is reversible and accompanied by simultaneous changes in multiple histone modifications. At the last step, maintenance of silencing becomes independent of Tsix and irreversible, which correlates with occupation of the -1 position of the transcription start site by a nucleosome and initiation of DNA methylation introduction. This study highlights the hierarchy of multiple chromatin events upon stepwise gene silencing establishment. |
| 巻・号 | 34(13) |
| ページ | 108912 |
| 公開日 | 2021-3-30 |
| DOI | 10.1016/j.celrep.2021.108912 |
| PII | S2211-1247(21)00226-6 |
| PMID | 33789104 |
| MeSH | Animals CCCTC-Binding Factor / metabolism DNA Methylation / genetics Epigenesis, Genetic Euchromatin / metabolism* Fibroblasts / cytology Fibroblasts / metabolism Gene Silencing Germ Layers / cytology Heterochromatin / metabolism* Histones / metabolism Mice Nucleosomes / metabolism Promoter Regions, Genetic* Protein Processing, Post-Translational RNA, Long Noncoding / genetics* RNA, Long Noncoding / metabolism Stem Cells / metabolism Transcription, Genetic* YY1 Transcription Factor / metabolism |
| IF | 8.109 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 21 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | EpiSC 129Ba1(AES0204) |