論文 - 詳細
| RRC ID | 64464 |
|---|---|
| 著者 | Montigny A, Tavormina P, Duboe C, San Clémente H, Aguilar M, Valenti P, Lauressergues D, Combier JP, Plaza S. |
| タイトル | Drosophila primary microRNA-8 encodes a microRNA-encoded peptide acting in parallel of miR-8. |
| ジャーナル | Genome Biol |
| Abstract |
BACKGROUND:Recent genome-wide studies of many species reveal the existence of a myriad of RNAs differing in size, coding potential and function. Among these are the long non-coding RNAs, some of them producing functional small peptides via the translation of short ORFs. It now appears that any kind of RNA presumably has a potential to encode small peptides. Accordingly, our team recently discovered that plant primary transcripts of microRNAs (pri-miRs) produce small regulatory peptides (miPEPs) involved in auto-regulatory feedback loops enhancing their cognate microRNA expression which in turn controls plant development. Here we investigate whether this regulatory feedback loop is present in Drosophila melanogaster. RESULTS:We perform a survey of ribosome profiling data and reveal that many pri-miRNAs exhibit ribosome translation marks. Focusing on miR-8, we show that pri-miR-8 can produce a miPEP-8. Functional assays performed in Drosophila reveal that miPEP-8 affects development when overexpressed or knocked down. Combining genetic and molecular approaches as well as genome-wide transcriptomic analyses, we show that miR-8 expression is independent of miPEP-8 activity and that miPEP-8 acts in parallel to miR-8 to regulate the expression of hundreds of genes. CONCLUSION:Taken together, these results reveal that several Drosophila pri-miRs exhibit translation potential. Contrasting with the mechanism described in plants, these data shed light on the function of yet undescribed primary-microRNA-encoded peptides in Drosophila and their regulatory potential on genome expression. |
| 巻・号 | 22(1) |
| ページ | 118 |
| 公開日 | 2021-4-23 |
| DOI | 10.1186/s13059-021-02345-8 |
| PII | 10.1186/s13059-021-02345-8 |
| PMID | 33892772 |
| PMC | PMC8063413 |
| MeSH | Animals Drosophila / genetics* Female Gene Expression Profiling Gene Expression Regulation* Male MicroRNAs / chemistry MicroRNAs / genetics* Mutation Nucleic Acid Conformation Open Reading Frames Peptides / genetics* Phenotype Protein Biosynthesis RNA Interference RNA, Long Noncoding |
| IF | 10.806 |
| リソース情報 | |
| ショウジョウバエ | DGRC#104917 |