RRC ID 64554
Author Izumi Y, Fujii K, Wien F, Houée-Lévin C, Lacombe S, Salado-Leza D, Porcel E, Masoud R, Yamamoto S, Réfrégiers M, Hervé du Penhoat MA, Yokoya A.
Title Structure Change from β-Strand and Turn to α-Helix in Histone H2A-H2B Induced by DNA Damage Response.
Journal Biophys J
Abstract Using synchrotron radiation-based circular dichroism spectroscopy, we found that the DNA damage response induces an increase of α-helix structure and a decrease of β-strand and turn structures in histone H2A-H2B extracted from x-irradiated human HeLa cells. The structural alterations correspond to the assumption that an average of eight amino acid residues form new α-helix structures at 310 K. We propose the structural transition from β-strand and turn structures to an α-helix structure in H2A-H2B as a novel, to our knowledge, process involved in the DNA damage response.
Volume 111(1)
Pages 69-78
Published 2016-7-12
DOI 10.1016/j.bpj.2016.06.002
PII S0006-3495(16)30399-X
PMID 27410735
Human and Animal Cells HeLa/Fucci(RCB2812)