論文 - 詳細
| RRC ID | 64580 |
|---|---|
| 著者 | Nakamura A, Kakihara Y, Funayama A, Haga K, Mikami T, Kobayashi D, Yoshida Y, Izumi K, Kobayashi T, Saeki M. |
| タイトル | HEATR1, a novel interactor of Pontin/Reptin, stabilizes Pontin/Reptin and promotes cell proliferation of oral squamous cell carcinoma. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Pontin and Reptin are closely related proteins belonging to the AAA+ (ATPases Associated with various cellular Activities) family. They form a hetero-oligomeric complex, Pontin/Reptin, which is involved in protein stability and assembly of the protein complexes as a molecular chaperone. Overexpression of Pontin and Reptin in tumor cells has been reported and is implicated in the development of various cancers. However, the molecular mechanism of Pontin/Reptin function in oral squamous cell carcinoma (OSCC) development remains unclear. Here, we identify HEAT repeat-containing protein 1 (HEATR1) as a novel binding factor of Pontin/Reptin. Functionally, HEATR1 stabilizes Pontin/Reptin and positively regulates OSCC cell proliferation by activating mTOR and pre-rRNA synthesis. We also find that HEATR1 expression is markedly upregulated in tumor region of OSCC tissue. Hence, we propose that HEATR1 is involved in the regulation of mTOR and ribosome biogenesis as a potential protein stabilizer of Pontin/Reptin in OSCC. |
| 巻・号 | 557 |
| ページ | 294-301 |
| 公開日 | 2021-6-11 |
| DOI | 10.1016/j.bbrc.2021.04.021 |
| PII | S0006-291X(21)00617-3 |
| PMID | 33894417 |
| MeSH | ATPases Associated with Diverse Cellular Activities / genetics ATPases Associated with Diverse Cellular Activities / metabolism* Carrier Proteins / genetics Carrier Proteins / metabolism* Cell Line, Tumor Cell Proliferation / genetics* DNA Helicases / genetics DNA Helicases / metabolism* Gene Expression Regulation, Neoplastic / genetics Genes, rRNA Humans Immunohistochemistry Minor Histocompatibility Antigens / genetics Minor Histocompatibility Antigens / metabolism* Mouth Neoplasms / genetics Mouth Neoplasms / metabolism* Protein Binding RNA Interference RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism* Real-Time Polymerase Chain Reaction Squamous Cell Carcinoma of Head and Neck / genetics Squamous Cell Carcinoma of Head and Neck / metabolism* TOR Serine-Threonine Kinases / metabolism Tandem Mass Spectrometry Up-Regulation |
| IF | 2.985 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HSC-3(RCB1975) HSC-4(RCB1902) SAS(RCB1974) |