RRC ID 64807
Author Chang W, Pedroni A, Hohendorf V, Giacomello S, Hibi M, Köster RW, Ampatzis K.
Title Functionally distinct Purkinje cell types show temporal precision in encoding locomotion.
Journal Proc Natl Acad Sci U S A
Abstract Purkinje cells, the principal neurons of cerebellar computations, are believed to comprise a uniform neuronal population of cells, each with similar functional properties. Here, we show an undiscovered heterogeneity of adult zebrafish Purkinje cells, revealing the existence of anatomically and functionally distinct cell types. Dual patch-clamp recordings showed that the cerebellar circuit contains all Purkinje cell types that cross-communicate extensively using chemical and electrical synapses. Further activation of spinal central pattern generators (CPGs) revealed unique phase-locked activity from each Purkinje cell type during the locomotor cycle. Thus, we show intricately organized Purkinje cell networks in the adult zebrafish cerebellum that encode the locomotion rhythm differentially, and we suggest that these organizational properties may also apply to other cerebellar functions.
Volume 117(29)
Pages 17330-17337
Published 2020-7-21
DOI 10.1073/pnas.2005633117
PII 2005633117
PMID 32632015
PMC PMC7382291
MeSH Action Potentials Animals Behavior, Animal Brain Central Pattern Generators / physiology Cerebellum / physiology Cluster Analysis Electrophysiological Phenomena Female Locomotion / physiology* Male Models, Animal Purkinje Cells / physiology* Spinal Cord Zebrafish / physiology*
IF 9.412
Zebrafish Tg(aldoca:BoTx-GFP)