RRC ID 64818
Author Ma W, Zhou J, Chen J, Carr AM, Watanabe Y.
Title Meikin synergizes with shugoshin to protect cohesin Rec8 during meiosis I.
Journal Genes Dev
Abstract The conserved meiosis-specific kinetochore regulator, meikin (Moa1 in fission yeast) plays a central role in establishing meiosis-specific kinetochore function. However, the underlying molecular mechanisms remain elusive. Here, we show how Moa1 regulates centromeric cohesion protection, a function that has been previously attributed to shugoshin (Sgo1). Moa1 is known to associate with Plo1 kinase. We explore Plo1-dependent Rec8 phosphorylation and identify a key phosphorylation site required for cohesion protection. The phosphorylation of Rec8 by Moa1-Plo1 potentiates the activity of PP2A associated with Sgo1. This leads to dephosphorylation of Rec8 at another site, which thereby prevents cleavage of Rec8 by separase.
Volume 35(9-10)
Pages 692-697
Published 2021-5-1
DOI 10.1101/gad.348052.120
PII gad.348052.120
PMID 33888556
PMC PMC8091969
MeSH Cell Cycle Proteins / metabolism* Chromosomal Proteins, Non-Histone / metabolism* Meiosis / genetics* Phosphoproteins / metabolism Phosphorylation / genetics Protein Serine-Threonine Kinases / metabolism Schizosaccharomyces / enzymology Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Separase / metabolism
IF 9.527