論文 - 詳細
| RRC ID | 64922 |
|---|---|
| 著者 | Ohto U, Ishida H, Krayukhina E, Uchiyama S, Inoue N, Shimizu T. |
| タイトル | Structure of IZUMO1-JUNO reveals sperm-oocyte recognition during mammalian fertilization. |
| ジャーナル | Nature |
| Abstract |
Fertilization is a fundamental process in sexual reproduction, creating a new individual through the combination of male and female gametes. The IZUMO1 sperm membrane protein and its counterpart oocyte receptor JUNO have been identified as essential factors for sperm-oocyte interaction and fusion. However, the mechanism underlying their specific recognition remains poorly defined. Here, we show the crystal structures of human IZUMO1, JUNO and the IZUMO1-JUNO complex, establishing the structural basis for the IZUMO1-JUNO-mediated sperm-oocyte interaction. IZUMO1 exhibits an elongated rod-shaped structure comprised of a helical bundle IZUMO domain and an immunoglobulin-like domain that are each firmly anchored to an intervening β-hairpin region through conserved disulfide bonds. The central β-hairpin region of IZUMO1 provides the main platform for JUNO binding, while the surface located behind the putative JUNO ligand binding pocket is involved in IZUMO1 binding. Structure-based mutagenesis analysis confirms the biological importance of the IZUMO1-JUNO interaction. This structure provides a major step towards elucidating an essential phase of fertilization and it will contribute to the development of new therapeutic interventions for fertility, such as contraceptive agents. |
| 巻・号 | 534(7608) |
| ページ | 566-9 |
| 公開日 | 2016-6-23 |
| DOI | 10.1038/nature18596 |
| PII | nature18596 |
| PMID | 27309808 |
| MeSH | Binding Sites / genetics Carrier Proteins / chemistry* Carrier Proteins / genetics Carrier Proteins / metabolism* Crystallography, X-Ray Egg Proteins Female Humans Immunoglobulins / chemistry* Immunoglobulins / genetics Immunoglobulins / metabolism* Ligands Male Membrane Proteins / chemistry* Membrane Proteins / genetics Membrane Proteins / metabolism* Models, Molecular Mutation Oocytes / chemistry Oocytes / metabolism Protein Binding / genetics Protein Structure, Tertiary Receptors, Cell Surface Sperm-Ovum Interactions* / genetics Spermatozoa / chemistry Spermatozoa / metabolism |
| IF | 42.779 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 59 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | COS-7(RCB0539) |